Article Text


Adverse pregnancy outcomes and subsequent risk of cardiovascular disease in women with systemic lupus erythematosus
  1. Pin Lin1,
  2. Elisa Rhew1,
  3. Roberta B Ness2,
  4. Alan Peaceman3,
  5. Alan Dyer4,
  6. David McPherson5,
  7. George T Kondos6,
  8. Daniel Edmundowicz7,
  9. Kim Sutton-Tyrrell8†,
  10. Trina Thompson8 and
  11. Rosalind Ramsey-Goldman1
  1. 1Division of Rheumatology, Department of Medicine, Northwestern University, Feinberg School of Medicine, Chicago, Illinois, USA
  2. 2Department of Epidemiology, The University of Texas, School of Public Health, Houston, Texas, USA
  3. 3Department of Obstetrics and Gynecology, Northwestern University, Feinberg School of Medicine, Chicago, Illinois, USA
  4. 4Department of Preventive Medicine, Feinberg School of Medicine, Chicago, Illinois, USA
  5. 5Division of Cardiology, Department of Medicine, University of Texas Health Science Center, Houston, Texas, USA
  6. 6Division of Cardiology, Department of Medicine, University of Illinois, Chicago, Illinois, USA
  7. 7Temple University School of Medicine, Philadelphia, Pennsylvania, USA
  8. 8Department of Epidemiology, University of Pittsburgh, Graduate School of Public Health, Pittsburgh, Pennsylvania, USA
  1. Correspondence to Rosalind Ramsey-Goldman; rgramsey{at}


Background/objective Patients with systemic lupus erythematosus (SLE) are at increased risk for adverse pregnancy outcomes and cardiovascular disease (CVD). The objective of this exploratory study was to investigate the association between a history of adverse pregnancy outcomes and subsequent risk of subclinical CVD assessed by imaging studies and verified clinical CVD events in 129 women with SLE.

Methods The occurrence of adverse pregnancy outcomes, specifically pre-eclampsia, preterm birth and low birth weight was ascertained by questionnaire. Subclinical CVD was assessed by coronary artery calcium (CAC) as measured by electron beam CT and carotid plaque measured by B mode ultrasound. Clinical CVD events were verified by medical record review. Logistic regression was used to estimate the association of pregnancy complications with occurrence of subclinical CVD and clinical CVD with a priori adjustment for age, which is associated with CVD and SLE disease duration as a measure of SLE disease burden.

Results Fifty-six women reported at least one pregnancy complication while 73 had none. Twenty-six women had at least one pregnancy complicated by pre-eclampsia and were more likely to have a CAC score greater than or equal to 10 (adjusted OR=3.7; 95% CI 1.2 to 11.9), but the presence of plaque was not associated with this pregnancy complication, OR=1.1, (95% CI 0.4 to 2.8). Low birth weight and preterm birth were not associated with CAC or plaque.

Conclusions Patients with SLE with a history of pre-eclampsia had a higher rate of subclinical CVD as measured by CAC score. Future studies are needed to confirm the relationship between adverse pregnancy outcomes and subsequent subclinical CVD and clinical CVD events.

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Key messages

  • Women with SLE and prior adverse pregnancy outcomes were more likely to have subsequent hypertension compared to those women with SLE and no prior adverse pregnancy outcomes.

  • Women with SLE and a history of pre-eclampsia had an almost four fold increase in the rate of subclinical cardiovascular disease as measure by coronary artery scores of 10 or greater.

  • Plaque score was not associated with prior adverse pregnancy outcomes in women with SLE.

Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterised by systemic inflammation affecting predominantly young premenopausal women. Compared with the non-lupus population, patients with SLE are at an increased risk for pregnancy related complications, including pre-eclampsia, preterm delivery and low fetal birth weight.1–3 As disease-related survival has improved over the past two decades, premature cardiovascular disease (CVD) is now recognised as a serious long-term complication contributing significantly to the morbidity and subsequent mortality of patients with lupus. Women with SLE experience CVD events at a higher rate, and at earlier ages compared with the general population.4–6

Pre-eclampsia is a pregnancy syndrome marked by de novo elevations in blood pressure and proteinuria. Studies in patients without lupus suggest that cardiovascular morbidity and mortality are increased among those who had previously experienced pre-eclampsia,7–11 preterm delivery9 ,11 ,12 and delivery of low birthweight infants11 ,13 ,14 when compared with women with uncomplicated pregnancies.15

Associations that link pregnancy complications to later life CVD are not well understood and include classic cardiovascular risk factors, inflammation and thrombosis15 which are all relevant to patients with SLE.16 What is not known is whether a relationship exists between pregnancy complications and CVD in women with SLE.

The objective of this study was to evaluate the association between a prior history of pregnancy complications and subsequent risk of CVD assessed by subclinical measured and verified events in women with SLE. Two measures of subclinical CVD associated with cardiovascular morbidity in the general population,17 ,18 coronary artery calcium (CAC) measured by ultrafast electron beam CT and carotid plaque using B mode carotid ultrasound have also been noted in women with SLE.19–21 Women with SLE participating in an ongoing prospective study assessing subclinical CVD and CVD events were queried on their history of pregnancy complications, including pre-eclampsia, preterm delivery and low birth weight.


Study population

The parent study, Study of Long-term Vascular and Bone Outcomes in Lupus Erythematosus (SOLVABLE), from which data for the current study are derived, is a longitudinal epidemiological study designed to assess the risk for subclinical and clinical CVD in 185 patients with lupus from the Chicago Lupus Database compared with 186 healthy controls.

Data collection

The SOLVABLE study and this exploratory study were approved by the Institutional Review Board at Northwestern University. All patients gave informed consent prior to their participation in these studies.

Demographic information, pregnancy-related information, traditional CVD risk factors and clinical CVD events were determined through interviews, self-report pregnancy questionnaires, physical examinations and serological tests. Adverse pregnancy outcomes ascertained included the following: (1) history of pre-eclampsia or history of pregnancy induced hypertension and proteinuria not due to lupus flare using clinical definitions,22 (2) preterm delivery (<37 weeks of gestation) and (3) full term low birth weight (<5.5 lbs).23 The adverse pregnancy outcomes were mutually exclusive and adjudicated in the following order: pre-eclampsia, then preterm delivery, then low birth weight if full term. All pregnancy data was provided by the patients and was verified if they were available from the medical records (labour and delivery procedure notes as well as clinical notes) at Prentice Women’s Hospital, Chicago, Illinois, USA. Traditional CVD risk factors included: (1) hypertension (defined as self-reported history of hypertension, systolic blood pressure ≥140, diastolic blood pressure ≥90 or taking antihypertensive medications), (2) current smoking, (3) family history of myocardial infarction (MI) in a first degree relative before the age of 60 years, (4) diabetes (defined as self-reported history of diabetes or fasting glucose ≥126 mg/dL), (5) hypercholesterolaemia (defined as self-reported history of high cholesterol or taking cholesterol-lowering medications). In addition, height, weight, waist and hip measurements were obtained.

To assess SLE-related characteristics, we used validated measures of lupus disease activity (Systemic Lupus Erythematosus Disease Activity Index-2000) and disease damage (Systemic Lupus Internatioanl Collaborating Clinics Index (SLICC-DI)) which were completed by trained physicians. SLICC-DI was modified to remove the three items related to CVD. Disease duration was calculated using the date the subject fulfilled the fourth American College of Rheumatology classification criteria for SLE.26 ,27 Renal disease was defined as being present if the subject had fulfilled American College of Rheumatology classification criteria for lupus renal involvement (>0.5 gm/day or 3 + proteinuria and/or the presence of cellular casts)26 ,27 or had a renal biopsy with evidence of WHO Class IIb, III, IV or V lupus nephritis.28 Participants provided information on corticosteroid treatment (current use, ever use and duration of treatment), as well as current use of hydroxychloroquine and immunosuppressants (cyclophosphamide, azathioprine, methotrexate, mycophenolate mofetil, ciclosporin and tacrolimus). Prior corticosteroid use and duration of treatment were verified by review of the subject's medical chart.

Measures of subclinical CVD were presence of carotid artery plaque and CAC score. Carotid artery plaque was imaged using carotid B mode ultrasound, which was performed at the Cardiovascular Imaging Processing Laboratory at Northwestern University and read at the University of Pittsburgh Ultrasound Research Laboratory. Carotid artery plaque was defined as a distinct area protruding into the vessel lumen that was at least 50% thicker than the surrounding areas and was measured at eight sites (bilateral internal carotid, external carotid, common carotid and carotid bulb). The outcome measure used for analysis was the presence or absence of plaque (plaque ≥1 vs plaque=0) as previously described by our group.29 The reproducibility of carotid duplex scanning using this technique has been previously documented in the Pittsburgh SLE cohort and a non-SLE population.30 ,31

CAC score was measured using electron beam CT (Imatron C-150 Ultrafast CT Scanner) at the University of Illinois, Chicago, and CAC scores were calculated with a densitometric programme available on the Imatron C-150 scanner, using the Agatston method.32 The outcome measures used for the analysis of CAC were the absence (CAC score <10) or presence (CAC score ≥10). The CAC scores were read at the University of Pittsburgh Cardiovascular Institute.

Clinical cardiovascular events assessed at the time of the baseline visit were (1) MI, (2) angina, (3) percutaneous transluminal coronary angioplasty, (4) coronary artery bypass surgery, (5) cerebrovascular accident and (6) transient ischaemic attack and were verified using the Multiethnic Study of Atherosclerosis protocol.33 All CVD events occurred after pregnancy and after the diagnosis of SLE.

Laboratory tests included fasting lipids (total cholesterol, low density lipoprotein cholesterol (LDLc), high density lipoprotein cholesterol and triglycerides), homocysteine, glucose and insulin, which were measured in the Lipid Laboratory at the University of Pittsburgh Graduate School of Public Health and Prevention. The Friedewald equation was used to estimate LDLc, unless the triglyceride level was >400. In that case, LDLc was measured directly. Plasma glucose levels were determined by enzymatic assay, and plasma insulin levels were measured by radioimmunoassay. Circulating inflammatory and endothelial markers measured included homocysteine, C reactive protein (CRP), intracellular adhesion molecule (ICAM), vascular cell adhesion molecule (VCAM) and E-selectin. Homocysteine was measured at the University of Pittsburgh Medical Center nutrition laboratory spectrophotometrically on the Olympus AU400 using reagents obtained from Carolina Liquid Chemistries (Brea, California, USA). The intra-assay and interassay coefficients of variation (CVs) are 2.8% and 6.3%, respectively. CRP, ICAM, VCAM and soluble E-selectin were measured at the University of Vermont. CRP was measured using the BNII nephelometer from Dade Behring using a particle enhanced immunonepholometric assay. Intra-assay CVs range from 2.3% to 4.4% and inter-assay CVs range from 2.1 to 5.7%. ICAM, VCAM and E-selectin were measured using ELISA assays (R&D Systems, Minneapolis, Minnesota, USA). The intra-assay and interassay CVs for VCAM range from 4.3–5.9% and 8.5–10.2%, respectively and the interassay CV for E-selectin range from 5.7% to 8.8%. For ICAM, the interassay laboratory CV is 5.0%. Antiphospholipid antibodies: anticardiolipin (ACL) antibodies (IgG and IgM; Diasorin, Stillwater, Minnesota, USA) and lupus anticoagulant (partial thromboplastin time or Russell's viper venom time with mix) were measured at the Coagulation Laboratory at University of Pittsburgh Medical Center. ACL IgG was considered positive if the result was >10 units and ACL IgM was considered positive if >15 units, as per laboratory standards.

Statistical methods

Descriptive statistics (eg, means, SDs, centiles, ranges) were used to determine distributions of the measures of subclinical CVD, cardiovascular risk factors (eg, smoking history, cholesterol, blood pressure) and inflammatory/endothelial markers. Differences between women with and without pregnancy complications were examined using t tests for normally distributed continuous variables, Wilcoxon's rank sum test for non-parametric comparison of continuous variables with skewed distributions and χ2 analyses or small sample methods for proportions. Logistic regression was used to estimate the associations of pregnancy complications with occurrence of subclinical CVD and clinical CVD, with a priori adjustment for age, which is associated with CVD and SLE disease duration as a measure of SLE disease burden. We also performed a sensitivity analysis substituting disease duration for disease damage using the SLICC-DI excluding the CVD-related variables as a measure of SLE disease burden in our logistic regression model. Since the results obtained were similar using either model, we present the results from the a priori model design which used disease duration as the measure of disease burden.


The clinical characteristics of the 185 SLE participants who were enrolled in the SOLVABLE parent study have been previously described.29 ,34 Of the 185 SLE participants, 51 had never been pregnant or only had elective terminations and 5 women refused to participate, leaving 129 subjects included in this exploratory study.

The women with adverse pregnancy outcomes were similar with respect to mean age at study visit (44.5±9.7 vs 44.4±9.7 years), current smoking, body mass index, waist-hip ratio, family history of MI, hypercholesterolaemia and diabetes, but more had hypertension (66% vs 47%, p=0.027) compared with women without adverse pregnancy outcomes (table 1). There were no differences in SLE characteristics (disease duration, medications, renal disease, ACL antibody status or Systemic Lupus Erythematosus Disease Activity Index-2000 score) or circulating markers of inflammation and endothelial activation in women with and without adverse pregnancy outcomes. In contrast, women with SLE and adverse pregnancy outcomes compared with those without adverse pregnancy outcomes had more SLE disease damage (SLICC-DI 2.1±1.8 vs 1.4±1.7, p=0.042) and menopause onset at an earlier mean age (37.4±8.0 vs 43.8±6.7, p=0.005), but the percentage of those who were postmenopausal at the baseline visit was similar, 38% vs 43% (table 1).

Table 1

Characteristics and traditional cardiovascular risk factors in patients with SLE (n=129) with and without history of adverse pregnancy outcomes*

There were 331 pregnancies in the 129 women participating in this study and 66% of the pregnancies occurred before the diagnosis of SLE. Of the 33% of pregnancies occurring after the diagnosis of SLE, the mean disease duration between SLE diagnosis and pregnancy was 6.8±6.0 years and 7.3±5.9 years in those without and with adverse pregnancy outcomes, respectively. Of the 56 women who reported adverse pregnancy outcomes, 26 had at least one pregnancy complicated by pre-eclampsia during pregnancy; 36 had at least one pregnancy resulting in preterm birth; and 28 had at least one low birthweight infant. Seventy-three women had no adverse pregnancy outcomes.

Two women with adverse pregnancy outcomes, one preterm and one with a low birthweight infant, were excluded from the analysis for CAC, but included in the analysis for the presence of plaque because the former measure was not available. A higher percentage of women with an adverse pregnancy outcome had a CAC score greater than or equal to 10 and the presence of plaque compared with women without an adverse pregnancy outcome, 25.9% vs 17.8% and 46.4% vs 38.4%, respectively. However these increases in the frequency of CAC score and the presence of plaque were not statistically significant after adjusting for age and SLE disease duration as noted in table 2. When evaluating the different types of adverse pregnancy outcomes individually, women with a history of pre-eclampsia were more likely to have a CAC score greater than or equal to 10 (adjusted OR=3.7, 95% CI 1.2 to 11.9), but not carotid plaque (adjusted OR=1.1, 95% CI 0.4 to 2.8) compared with those without adverse pregnancy outcomes. The ORs for CAC score greater than equal to 10 and the presence of carotid plaque for women with at least one pre-term birth and low birth weight were not statistically significant as noted in table 2.

Table 2

Association between subclinical cardiovascular disease imaging of the coronary and carotid arteries in patients with SLE with and without adverse pregnancy outcomes

Clinical cardiovascular events occurred in four (7.14%) women with pregnancy complications and in six (8.22%) without pregnancy complications (OR=0.6, 95% CI 0.2 to 3.2). Of the four women with pregnancy complications, three had transient ischaemic attacks and one had percutaneous transluminal coronary angioplasty and of the six women without pregnancy complications four had cerebrovascular accidents, one had a MI, one had a MI and percutaneous transluminal coronary angioplasty.


Women with SLE have been reported to have more pregnancy complications,1–3 as well as a higher cardiovascular risk.16 This report describes the relationship between adverse pregnancy outcomes and subsequent subclinical CVD and CVD events in women with SLE. In this study, women with a history of pre-eclampsia had almost a fourfold increased risk of having subsequent subclinical CVD based on the presence of CAC score greater than or equal to 10.

One factor that may underlie the relationship between pregnancy complications and CVD is that pregnancy complications, such as pre-eclampsia, low birth weight and preterm delivery share several common risk factors with CVD, including obesity, cigarette smoking, hypertension, diabetes and lipid abnormalities35–43 as recently reviewed by Rich-Edwards and colleagues.15These traditional CVD risk factors are associated with an increased risk of developing pregnancy complications, and conversely, pregnancy complications are also associated with an increased susceptibility for the subsequent development of traditional CVD risk factors, including hypertension, increased insulin resistance, obesity and lipid abnormalities.9 ,11 ,44–46 In our study, we also found a significant association between a history of pregnancy complications and hypertension (p=0.027, table 1), as well as an association for diabetes (p=0.088, table 1). However, we did not distinguish between type 1 and type 2 diabetes in this study. Similar associations between cardiovascular risk factors and pre-eclamspia have also been noted in women with polycystic ovarian syndrome, another population of women with premature atherosclerosis.47

Recent studies have demonstrated that women with pre-eclampsia continue to have adverse metabolic and vascular abnormalities even years after delivery despite normalisation of blood pressure. When examined years after their affected pregnancy, women with a history of pre-eclampsia have worse lipid profiles,48 more hyperinsulinemia49 and more microalbuminuria50 than women with normotensive pregnancies. In addition, women with a history of pre-eclampsia had impaired endothelial function when examined 3 years after their pre-eclamptic episode.51 In women with SLE, there may be lupus-related factors such as autoantibodies that are contributing to vascular abnormalities in the endothelium as possible underlying risk factors for developing pre-eclampsia and CVD,52 however we were not able to demonstrate such an association with the autoantibodies measured in this study, likely due to our limited sample size and cross-sectional study design.

Since inflammation plays a role in atherosclerosis53 and SLE54 and the inflammatory response is altered in pre-eclampsia compared with normal pregnancies, with elevated levels of cytokines and vascular adhesion molecules,55–58 it portends a potentially detrimental combination for the future risk of CVD in women with SLE. Furthermore, inflammation has also been implicated in preterm birth59 and fetal growth restriction.12 ,60

The inflammatory response is triggered as the result of endothelial damage caused by risk factors such as hypertension, diabetes and cigarette smoking. Consequently, there is activation of inflammatory cells which release cytokines, chemokines and growth factors, as well as altered endothelial function. Propagation of the ensuing inflammatory response may result when exposures to these injurious factors persist or if the immune system becomes dysregulated. The physiological maternal inflammatory responses become exaggerated in pre-eclamptic pregnancy compared with normal pregnancy61 irrespective of SLE. Elevated levels of tumour necrosis factor α and interleukin 6, as well as adhesion molecules, VCAM and E-selectin46 ,56 ,58–60 ,62–64 have been found in pregnancies complicated by pre-eclampsia, low birthweight infants and preterm births compared with normal pregnancy. In this study we were not able to document an association between inflammatory markers or adhesion molecules likely due to the small sample size and the cross-sectional nature of the study design.

The results from this study have some additional limitations. We also evaluated possible misclassification errors. The number of subjects in this study was small compared with the numbers studied in the general population evaluating associations between pregnancy complications and subclinical CVD and events which limit the precision of our results. Maternal recall regarding certain pregnancy details have been shown to be reliable many years postpartum.65 We verified the self-reported pregnancy data for those subjects who had given birth at Prentice Women’s Hospital, Chicago, Illinois and found 100% accuracy using a similar strategy in a previous study,66 so maternal recall is not likely to be a problem for self-reported adverse pregnancy outcomes. Although we verified pregnancy outcomes, we were not able to document smoking or alcohol history at the time of pregnancy. We verified cardiovascular events, but we do not have biological samples nor did we perform detailed clinical assessments of SLE disease activity and medications used or tested for autoantibodies, inflammatory or endothelial markers during pregnancy, at the time subclinical CVD disease was assessed or when the cardiovascular events occurred. This study did not enrol and follow subjects prior to pregnancies. This precludes evaluation of autoantibodies, inflammatory markers and endothelial activation markers prior to pregnancy and a possible role in the occurrence of adverse pregnancy outcomes. The cross-sectional study design does not permit us to assess causality between autoantibodies, inflammatory markers, and endothelial activation markers, pregnancy outcomes and subclinical CVD and clinical CVD events nor can we eliminate the possibility that covariates measured after pregnancy could have changed over time and could also be confounders influencing pregnancy outcomes. We controlled for SLE duration in our models and performed a sensitivity analysis substituting disease damage measured by the modified SLICC-DI (data not shown) for disease duration with similar results, making it less likely that SLE was acting as a mediating factor for the occurrence of pregnancy outcomes.

In summary, patients with SLE with a history of pre-eclampsia had an almost fourfold increase in the rate of subclinical CVD as measured by CAC score greater than or equal to 10, but the presence of plaque was not associated with this adverse pregnancy outcome. Neither low birth weight nor preterm birth was associated with CAC or plaque. The results reported here are preliminary. To explore these findings further larger, prospective studies are needed to examine the incidence of subclinical CVD and clinical CVD events in women with SLE who have a history of adverse pregnancy outcomes similar to observations in the general population15 and also other groups with premature CVD such as women with polycystic ovarian syndrome.47

  • Received January 30, 2014.
  • Revision received April 22, 2014.
  • Accepted April 28, 2014.


View Abstract


  • PL and ER are co-primary authors.

  • Deceased.

  • Acknowledgements The authors thank Dr Malena Law for assistance with pregnancy outcomes, Dr Zoreh Davanipour for editorial assistance, and Beverly Smulevitz and Bonnie Kane for help in carotid plaque data collection and evaluation. The authors also thank the University of Pittsburgh and the University of Vermont for collaborative efforts on laboratory analyses.

  • Funding This work was supported by grants from the National Institutes of Health (NIH) T32-AR07611, F32-AR51681, K23-AR054418, and Mary Kirkland Center for Lupus Research and Rheuminations, (ER); NIH T32-AR07611, F32-HL082224 (PL); NIH K24-AR02318, P60-AR30692, P60-AR48098, NCRR/GCRC M01-RR00048 (RR-G).

  • Competing interests None.

  • Patient consent Obtained.

  • Ethics approval Illinois.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data sharing statement Any investigator who wishes to obtain data from this Project will do so with a Resource Sharing Agreement that provides for (1) a commitment to using the resources only for research purposes and not to identify an individual participant, (2) a commitment to securing resources using appropriate computer technology, and (3) a commitment to destroy or return the resources after analyses are completed. There is no additional unpublished data from the pregnancy project from SOLVABLE.

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