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274 Prevalence of anti-dfs70 antibodies in a colombian cohort: a case-control study
  1. S Arteaga1,
  2. T Urrego2,
  3. LM Peñaranda3,
  4. A Londoño4,
  5. ML Cardenas4,
  6. AL Vanegas1,
  7. CH Muñoz1,
  8. M Escobar1,
  9. W Rojas1,
  10. G Vásquez1,2,
  11. LA González1,
  12. C Perez Koller3 and
  13. JA Gómez-Puerta1,2,4
  1. 1Universidad de Antioquia, Sección de Reumatología, Medellin, Colombia
  2. 2Universidad de Antioquia, Grupo de Inmunología Celular e Inmunogenética, Medellin, Colombia
  3. 3Werfen, Inova Diagnostics, Bogotá, Colombia
  4. 4Dinamica IPS, Autoinmunidad, Medellín, Colombia

Abstract

Background and aims Anti-dense fine speckled 70 (anti-DFS70) antibodies were initially identified as an ANA IIF pattern from patients with interstitial cystitis; however, some recent studies showed that anti-DFS70 antibodies are common among ANA positive individuals with no evidence of systemic autoimmune disease (SAD)(Mahler M. 2012). Information of anti-DFS70 in Latin-American countries is very limited. We determined the prevalence of Anti-DFS70 antibodies in a Colombian cohort.

Methods We evaluated individuals≥18 years old, including 100 SLE patients, 102 SADs, 200 healthy controls, and 56 subjects suspected of having autoimmune disease with ANA positive and negative anti ds-DNA antibodies. The presence of anti-DFS70 antibodies was determined by QUANTA Flash by chemiluminescent techniques (Inova/Werfen, San Diego)

Results Our final cohort included 458 samples. The mean age of SLE patients was 33±12 years, for SADs was 41±19 and for healthy controls was 36±10 years. The main diagnoses of SAD were: Vasculitis (n=28), RA (n=21), Systemic sclerosis (n=12), primary antiphospholipid syndrome (n=11), dermatomyositis (n=10) among others. Racial/ethnic breakdown was: 76% Mestizo and 20% Afro-latin Americans. Anti-DFS70 antibodies were positive in 1.8% of subjects with ANAs positive/anti DNA negative, in 1% of SLE patients, 0.9% of patients with other SADs and in 0.5% of healthy controls. Given the low prevalence of anti-DFS70 antibodies, no clinical correlations were possible.

Conclusions Despite anti-DFS70 antibodies are a good diagnostic tool for discrimination among healthy individuals and SADs (including SLE), we found a very low prevalence of anti-DFS70 antibodies in our Colombian cohort.

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