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342 New possibilities of human complement system in diagnostics and analyses of autoimmune diseases
  1. V Lakhtin1,
  2. M Naxthn2,
  3. S Andina1,
  4. S Afanasiev1,
  5. O Logunov1 and
  6. V Aleshkin1
  1. 1G.N. Gabrichevsky research Institute for Epidemiology and Microbiology, Department of Biotechnology, Moscow, Russia
  2. 2G.N. Gabrichevsky research Institute for Epidemiology and Microbiology, Department of Biotechology, Moscow, Russia

Abstract

Background and aims The aim was to summarise own data on the human complement system involving cofunctioning complement and other innate protective systems against infectious and autoimmunity diseases.

Methods Components of the patient serum complement (CPSC) were registered by immunochemical methods in microplates (variants of functional analyses of isotypes C4A and C4B, and C1-inhibitor upon supramolecular assembling on well bottom) and on the blot (preliminary isoelectrophoresis of sera in the plate of polyacrylamide gel). Rabbit and goat polyclonal antibodies against purified CPSC were used. Activity of antibodies-peroxidase conjugates bound to CPSC was detected in the presence of TMB (microplate) or chemiluminescent substrate in a real time [BioChemi System (UVP)].

Results 1.Sera of patients having autoimmune diseases were characterised on the blot by appearance of aggregated C4B and C4A within pI 4,0–4,7 compared to less acidic free isotypes. Functional status of isotypes was confirmed in microplate. Absolute amounts of isotypes and their subisotypes as well as ratio of isotypes characterised prognostic-diagnostic patient groups of diseases (SLE, antiphospholipid syndrome, rheumatoid arthritis). Appearance and relative intensities of the system of aggregated isotypes and subisotypes of C4 indicated the presence of disease, its initiation, reached phase of disease and disease character. 2.Similar localization on the blot for the complex C4B and C1-inhibitor of patients was registered.

Conclusions Results indicate possible cofunctioining C4B and C1-inhibitor in protection complement network upon development of autoimmune diseases. New mechanisms of cascade protection involving new combinations of CPSC may be revealed. Results open new practical possibilities in diagnostics of early, progressive and chronic autoimmune diseases.

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