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343 Complement lectin system cofunctions to other protective pro-tein systems involving relationships between lectins and glycoconjugates against autoimmune and infectious diseases
  1. M Lakhtin,
  2. V Lakhtin,
  3. S Andina,
  4. S Afanasiev and
  5. V Aleshkin
  1. G.N. Gabrichevsky research Institute for Epidemiology and Microbiology, Medical Biotechnology, Moscow, Russia

Abstract

Background and aims Innate protection recognising systems of human organism are important and perspective in respect of investigation autoimmune and infectious diseases. The aim was to evaluate lectin system (LS) of complement and its importance for innate interactome against infectious and autoimmune diseases.

Methods Patient complement components (PCC) of sera were estimated by immunochemical methods in microplates (hybrid plate for C4A and C4B functionality testing) and on the blot (for PCC separated by isoelectrophoresis). Peroxidase activity was detected using TMB or chemiluminescent substrate (for blot, BioChemi System, UVP).

Results Additional glycoconjugates(GC)-binding PCC were registered.

C1-inh revealed affinity to heparin. Patient (SLE, antiphospholipid syndrome, rheumatoid artritis) subisotypes of C4B (up to 5) and C4A (up to 7) were observed as aggregated forms together with GC.

Conclusions 1. Extended complement LS includes MBL, Factor H, C1-inh, CR1, CR2, CR3, C3, C4B, others. 2. Complement serve as a universal communicator among protective systems involving their LS—GC communications. 3. Complement (as mostly advanced innate protection system) possesses structure-function principles prognostic for any innate recognition systems. 4. There is superLS network in organism. Probiotic LS is important cofunctioning part of it. 5. The data support idea that any protection protein system partially functions involving LS—GC recognition (also for antibodies recognised by Fc-receptors as LS). 6. New prognostic-diagnostic possibilities in investigation of autoimmune and infectious diseases are opened using interactome LS—GC network.

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