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86 Mesenchymal stem cells induce lymphocytes apoptosis independent of bim and bcl-xl in lupus mice
  1. S Huang1,
  2. D Wang2 and
  3. L Sun1
  1. 1Drum Tower Clinical Medical College of Nanjing Medical University, Department of Immunology and Rheumatology, Nanjing, China
  2. 2Affiliated Drum Tower Hospital of Nanjing University Medical School, Department of Immunology and Rheumatology, nanjing, China

Abstract

Background and aims Mesenchymal stem cells (MSCs) have recently been used successfully in humans to control a lot of diseases. However, the mechanisms involved in their immunomodulatory effects remain a matter of debate. Here we explored whether lymphocytes apoptosis involved in the therapeutic effects of UC-MSCs in lupus mice.

Methods 1^ 106 of human UC-MSCs were injected into B6.lpr mice via tail vein and 6, 24 hours and 4 weeks later, all the mice were sacrificed, the apoptosis of lymphocyte in peripheral blood and spleen tissues as well as the expressions of Bim and Bcl-xl were detected by FACS, the immune cell subpopulations and cytokines in serum were also examined at 6 and 24 hours, respectively. The curative effects were assessed 4 weeks later.

Results UC-MSCs ameliorated disease progression of lupus mice at 4 weeks, increasing the percentage of Treg while downregulating Tfh, plasma cells and Th1 cells, decreasing spleen weight and repairing kidney lesion. UC-MSCs promoted lymphocyte apoptosis in peripheral blood and spleen at 6 and 24 hours, and reduced serum TGF-β1 levels, but did not affect Bim and Bcl-xl expressions in CD4+ and CD8+ T cells. Meanwhile, the percentage of Treg was significantly increased in the MSCT group at both 6 and 24 hours. Reductions in the proportions of plasma cells, Th1, Th2 cells were also evident at 24 hours after MSCs infusion.

Conclusions UC-MSCs exhibit extensive pro-apoptosis properties against lymphocytes in B6.lpr mice, which may offer a form of immunomodulatory therapy for lupus.

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