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Thursday 06 October 2022 from 13:00 to 14:10
Po.1 E- poster session 1: animal models, omics in lupus, major organ involvement inclucing npsle, organ damage and survival
PO.1.10 Multicentre study in sle patients with and without renal involvement by label free proteomic analysis of 24h urine
  1. N Rivera1,
  2. E Ruiz2,
  3. J Calvo3,
  4. E Aurrecoechea4,
  5. L Riancho4,
  6. A De Diego-Sola5,
  7. M Diaz6,
  8. E Galindez2,
  9. O Fernandez1,
  10. I Torre1,
  11. E Cuende1,
  12. J Blanco1,
  13. A Intxaurbe1,
  14. R Exposito1,
  15. C Garcia1,
  16. L Vega1,
  17. M Enjuanes2,
  18. M Allande1,
  19. F Elortza7,
  20. I Iloro7,
  21. M Azkargorta7 and
  22. M García-Vivar2
  1. 1ISS BIOCRUCES BIZKAIA ~ BARAKALDO ~ Spain
  2. 2Basurto University Hospital ~ Bilbao ~ Spain
  3. 3Araba University Hospital ~ Vitoria ~ Spain
  4. 4Sierrallana Hospital ~ Torrelavega ~ Spain
  5. 5Donostia University Hospital ~ Donostia ~ Spain
  6. 6Fundació PuigVert ~ Barcelona ~ Spain
  7. 7CIC bioGUNE ~ DERIO ~ Spain

Abstract

Background Lupus nephropathy (LN) is an important cause of morbidity and mortality in patients with Systemic Lupus Erythematosus (SLE). Considering that renal biopsy is a specialized technique and not risk free, a proteomics study is proposed to determine biomarkers that may help us to differentiate patients diagnosed with SLE with and without renal involvement.

Methods Prospective and multicentric study of proteomics was conducted in 24-hour urine from SLE patients with and without renal involvement, by label free nLC MS/MS analysis.

Results 124 patients were recruited from 5 centers: 49 patients with SLE and renal involvement and 73 patients with SLE without renal involvement. There were no differences between groups according to race, gender and age (Table 1).

A total of 718 proteins (identified with at least two peptides with a FDR<1%) were quantified and further considered in the analysis. The Student’s T-test analysis reflected the differential presence of 518 proteins (p<0.01) between patients with and without renal involvement, being 58 more abundant in the urine of the patients with renal damage, whereas 460 showed the opposite pattern. Two diagrams (diagram 1 & 2) by biological process and protein class, show the results.

Conclusions In this multicentric study, a different protein pattern in urine (over or under expression) is observed between patients with and without renal involvement. It is necessary to continue with the study of the results in the context of cell biology, to know the basis of the deregulation of the proteins found among these groups of patients. On the other hand more studies are needed to know if proteomics analysis of urine could serve as diagnostic/prognostic tool of lupus patients with and without renal involvement.

Abstract PO.1.10 Figure 1
Abstract PO.1.10 Figure 1
http://creativecommons.org/licenses/by-nc/4.0/

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

https://doi.org/10.1136/lupus-2022-elm2022.44

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