RT Journal Article
SR Electronic
T1 306 Unique association of hla-drb1*15, hla drb1*03 and ebv with adult and paediatric systemic lupus erythematosus (sle) patients in north india
JF Lupus Science & Medicine
JO Lupus Sci & Med
FD Lupus Foundation of America
SP A139
OP A139
DO 10.1136/lupus-2017-000215.306
VO 4
IS Suppl 1
A1 P Prabir Das
A1 RW Minz
A1 S Anand
A1 A Sharma
A1 B Saikia
A1 S Singh
YR 2017
UL http://lupus.bmj.com/content/4/Suppl_1/A139.1.abstract
AB Background and aims SLE is multisystem autoimmune disease, having known HLA-DRB1*15 and DRB1*03 risk alleles and an association with EBV in the Caucasoid population. We compared the association of HLA-DRB1 and EBV in North Indian cohort of adult and paediatric SLEMethods We analysed 109 adult SLE (aSLE) and 52 paediatric SLE (pSLE) with 278 age and sex matched control adult (CA) and paediatric (CP) for HLA-DRB1 genotyping by PCR-SSP, EBV-IgM and IgG to VCAgp125, VCAp19, EBNA-1, p22 and EA-D by line blot assay and EBV load by real-time PCRResults The frequencies of DRB1*15 and DRB1*03 were higher in SLE (OR=2.57 and 1.67 respectively) compared to controls. aSLE had higher frequencies of DRB1*03 (OR=2.33) and DRB1*04 (OR=7.51) compared to pSLE whereas, pSLE had higher frequency of DRB1*15 (OR=2.42) compared to aSLE. pSLE had more 3+ to 4+ positivity of EBV-IgG to VCAgp125 compared to aSLE (p=0.0008). EBV-IgM to VCAp19, EBNA-1 and EA-D (25%,12.5% and 6.25% respectively) were present only in pSLE. pSLE had higher EBV load compared to aSLE (p=0.045). IgG to p22 and EA-D were associated with DRB1*03 in aSLE (OR=3.92 and 5.28 respectively) and IgG to VCAgp125, VCAp19, EBNA-1, p22 and EA-D were associated with DRB1*15 in pSLE (OR=4.0,3.44,4.6,4.8 and 4.8 respectively)Conclusions DRB1*15 and DRB1*03 are risk alleles in North Indian SLE. Both show strong associations with immune response to EBV proteins. pSLE has stronger association with DRB1*15, which is associated with early infection, stronger immune response to EBV proteins and higher EB viral load, which may explain more severity of pSLE