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Evaluation of soluble tumor necrosis factor (TNF) receptors and TNF receptor antibodies in patients with systemic lupus erythematodes, progressive systemic sclerosis, and mixed connective tissue disease

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Abstract

Two TNF binding proteins have been characterized as soluble fragments of TNF receptors. We measured the plasma concentrations of soluble type A (p75) and type B (p55) TNF receptors in patients with systemic lupus erythematodes (SLE), progressive systemic sclerosis (PSS), and mixed connective tissue disease (MCTD). In SLE and PSS patients plasma concentrations of both types of TNF receptors and in MCTD patients type A TNF receptors were significantly elevated compared to controls. Plasma concentrations of both soluble TNF receptors were highly correlated in SLE, PSS, and MCTD patients, indicating a possible coregulation of both TNF receptors. In contrast, soluble interleukin 2 receptor (sCD 25) plasma concentrations were not correlated and seem to be an independent parameter. The soluble forms of the TNF receptors neutralize TNF in cytotoxicity assays and are functionally active as TNF antagonists. In one patient with SLE, autoantibodies against type A TNF receptors were detected, TNFα, and TNFβ did not interfere with the autoantibody binding to the receptor.

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Authors and Affiliations

  1. Medizinische Klinik und Poliklinik V der Universität Heidelberg, Hospitalstr. 3, 69 Heidelberg, Germany

    Bernhard Heilig, Christoph Fiehn, Antonio Pezzutto & Werner Hunstein

  2. Hoffmann La Roche AG, 4002, Basel, Switzerland

    Manfred Brockhaus & H. Gallati

Authors
  1. Bernhard Heilig
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  2. Christoph Fiehn
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  3. Manfred Brockhaus
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  4. H. Gallati
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  5. Antonio Pezzutto
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  6. Werner Hunstein
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Heilig, B., Fiehn, C., Brockhaus, M. et al. Evaluation of soluble tumor necrosis factor (TNF) receptors and TNF receptor antibodies in patients with systemic lupus erythematodes, progressive systemic sclerosis, and mixed connective tissue disease. J Clin Immunol 13, 321–328 (1993). https://doi.org/10.1007/BF00920240

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