Cell
Volume 46, Issue 5, 29 August 1986, Pages 717-724
Journal home page for Cell

Article
Visualization of the bent helix in kinetoplast DNA by electron microscopy

https://doi.org/10.1016/0092-8674(86)90347-8Get rights and content

Abstract

Kinetoplast DNA minicircles from the trypanosomatid Crithidia fasciculata contain a segment of ∼200 bp which is probably more highly bent than any other DNA previously studied. Electron microscopy (EM) of relaxed minicircles (2.5 kb) revealed 200–300 bp loops within the larger circles, and the loops could also be detected on full-length linear molecules. Examination by EM of a 219 bp cloned fragment which contains the bent helix revealed that up to 70% of the molecules appeared circular whether or not the ends were cohesive. In contrast, a 207 bp fragment from pBR322 showed no circles and the fragments in general appeared much straighter than the kinetoplast fragments. Treatment of the 219 bp bent kinetoplast fragment with the drug distamycin caused a striking reduction in curvature.

References (30)

  • J.M. Sperrazza et al.

    Electron microscopy can be used to measure DNA supertwisting

    Gene

    (1984)
  • L. Birkenmeyer et al.

    The majority of minicircle DNA in Crithidia fasciculata strain CF-Cl is of a single class with nearly homogeneous DNA sequence

    Nucl. Acids. Res.

    (1985)
  • S. Diekmann

    Sequence-specificity of curved DNA

    FEBS Lett.

    (1986)
  • P.T. Englund et al.

    The molecular biology of trypanosones

    Ann. Rev. Biochem.

    (1982)
  • G.W. Gough et al.

    DNA bending induced by cruciform formation

    Nature

    (1985)
  • Cited by (0)

    Present address: Houghton Poultry Research Station, Houghton, Huntingdon, Cambridgeshire PE17 2DA, United Kingdom.

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