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LP-085 Investigating the therapeutic potential of achatina fulica snail mucus on pristane-induced lupus nephritis in mice
  1. Arief Nurudhin1,
  2. Yulyani Werdiningsih1,
  3. Idhil Akbar2,
  4. Fatichati Budiningsih3,
  5. Nurhasan Agung Probowo1,
  6. Arfin Arifin1 and
  7. Indrayana Sunarso1
  1. 1Rheumatology Division Internal Medicine Department, Medical Faculty Sebelas Maret University, Indonesia
  2. 2Resident of Internal Medicine, Medical Faculty Sebelas Maret University, Indonesia
  3. 3Geriatric Department of Internal Medicine, Medical Faculty Sebelas Maret University, Indonesia


Background Administration of cytotoxic and immunosuppressive agents are well established treatment of systemic lupus erythematosus. However, the usage of these drugs shows frequent side effects. Therefore, finding new treatment option are necessary. Mucus secretion from snail (Achatina Fulica) contain glycosaminoglycans including heparan and acharan sulfate, which known to accelerate the inflammatory process and replace the damaged glomerular filtration membrane in renal interstitial fibrosis. This study aimed to determine the effect of snail mucus on levels of heparan sulfate in mice model of lupus nephritis.

Methods Experimental study uses posttest-only group design. The control group was male Balb/C mice injected with 0.5 cc NaCl 0.9% I.P, while mice model of nephritis lupus injected with 0.5 cc Pristane I.P. Nephritis lupus mice grouped into group I (received oral methylprednisolone 0.5mg/kgBW/day), group II (received oral 0.5 cc snail mucus/day) and group III (combination of standard therapy and snail mucus). The treatment administered for 4 weeks. Detection of heparan sulfate on blood serum taken 4 months after therapy. Statistical analysis used anova test and post hoc test.

Results There was an increase in heparan sulfate levels in the snail mucus group (12.13 +1.27 mg/dL; p = 0.277), and the methylprednisolone group (11.79 +0.97 mg/dL; p = 0.230) compared to the lupus group (11.27+1.20 mg/dL). The snail mucus group also showed higher level of heparan sulfate compared to control group, although not statistically significant (p = 0.257).

Conclusions Achatina fulica mucus may increase heparan sulfate level in mice model of lupus nephritis.


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  • Nephritis Lupus
  • Heparan Sulfate
  • Achantina fulica

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