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LP-144 Cytosolic localization of antibodies in monocytes is positively associated with disease activity of the patients with systemic lupus erythematosus
  1. Minjae Kim1,
  2. Youngsil Seo1,
  3. Juho Choi1,2,
  4. Yerin Jeon1,2,
  5. Young-In Noh1,2,
  6. Eunbin Lee1,2,
  7. Ju-Yang Jung3 and
  8. Myung-Hee Kwon1,2
  1. 1Department of Microbiology, Ajou University School of Medicine, 206 World cup-Ro, Yeongtong-Gu, Suwon, 16499, Republic of Korea
  2. 2Department of Biomedical Sciences, Graduate School, Ajou University, 206 World cup-Ro, Yeongtong-Gu, Suwon, 16499, Republic of Korea
  3. 3Department of Rheumatology, Ajou University School of Medicine, 206 World cup-Ro, Yeongtong-Gu, Suwon, 16499, Republic of Korea


Background Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease characterized by production of autoantibodies. The anti-double stranded DNA (anti-dsDNA) autoantibodies are diagnostic biomarkers of SLE. A subset of anti-dsDNA antibodies can enter living cells and induce cytokine secretion. Our previous studies have reported that internalizable anti-DNA IgG localize to the cytosol and trigger secretion of IL-8 and TNF-a in primary human CD14+ monocytes. In this study, we detected the presence of antibodies that localized to the cytosol in monocytes obtained from the patients with SLE.

Methods Cytosolic localization of IgG in CD14+ monocyte gated from PBMC of healthy controls (n=65) and SLE patients (n=160) was analyzed using a FACSCanto II flow cytometer. The Alexa Fluor 488 fluorescence intensity resulting from Igs existence (κ and λ chain of Ig) within the CD14+ monocyte population was then quantified as the mean fluorescence intensity (MFI). The presence of cytosolic IgG was determined by the ratio of permeabilization (P) to non-permeabilization (NP). [formula: Ratio of P:NP = (P – Control P)/(NP – Control NP)]

Results We show a statistically significant association between cytosolic localization of antibodies (ratio of P:NP) and disease activity in the patients with SLE. Compared with healthy controls (MFI=1.082), SLE patients had higher levels in active (MFI=1.245) and inactive (MFI=1.328) disease.

Conclusions Antibodies in the patients with SLE highly localize to the cytosol of monocytes, depending on the disease activity defined by SLE disease activity index (SLEDAI).

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