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Oral Presentation
Concurrent session 10: immune cells
LO-039 Vitamin D3 mitigates immune system alterations caused by activation of myeloid dendritic cells in SLE
  1. Mingfang Li1,
  2. Li Luo2,
  3. Chuanchuan Lin2,
  4. Bing Ni3,
  5. Liyun Zou3,
  6. Zhiqiang Song2,
  7. Fei Hao2 and
  8. Na Luo2
  1. 1Dermatology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China, China
  2. 2Dermatology, Southwest Hospital, Army Medical University, Chongqing, China
  3. 3Immunology, Institute of Immunology, PLA, Army Medical University, Chongqing, China

Abstract

Background Systemic lupus erythematosus (SLE) is an autoimmune disease in which defective T cells, immune complex deposition, and other immune system alterations contribute to pathological changes of multiple organs and organ systems. Our aim was to investigate the effects of 1,25-(OH)2 vitamin D3 (VitD3) on the activation of myeloid dendritic cells (mDCs) by autologous DNA immune complex (DNA-IC), and the effects of VitD3 on immune system balance during SLE.

Methods We purified DNA-ICs from SLE patients and isolated peripheral blood mDCs before and after histone deacetylase3(HDAC3) gene interference by siRNA. mDC was stimulated by DNA-ICs and/or VD3.The expression of NF-κB subunit RelB detected by WB. TNF-α,IL-10 secretion was detected by ELISA respectively. The immune balance of Treg/Th17 cells was determined after the co-culture of homologous CD4+T lymphocytes with different stimulators primed-mDCs.

Results Our in vitro studies indicated that DNA-ICs were internalized and consumed by mDCs. Further analysis indicated that VitD3 blocked the effects of DNA-ICs on RelB, IL-10, and TNF-α in mDCs. Co-culture of mDCs and CD4+T cells indicated that VitD3 inhibited multiple processes mediated by DNA-ICs, including the proliferation of mDCs, downregulation of IL-10, and upregulation of TNF-α. Additional co-culture experiments indicated that VitD3 reversed the effects of DNA-ICs in regulating the percentages of CD4+CD127-Foxp3+ T cells and CD4+IL17+ T cells.

Conclusions Our results indicated that autologous DNA-ICs stimulated activation of mDCs during SLE, and that VitD3 inhibited the stimulatory effects of DNA-ICs and maintained the Treg/Th17 immune cell balance. These results suggest that VitD3 may have therapeutic value for treatment of SLE.

http://creativecommons.org/licenses/by-nc/4.0/

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

https://doi.org/10.1136/lupus-2023-KCR.40

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