Article Text

Download PDFPDF

AI-22 Single cell protein and transcriptional profiling of CD4+ follicular B helper T (TFH) and central memory (TCM) cells in SLE: physiological and pathological phenotypes
  1. Minsuk Kwak1,
  2. Jin-Young Choi2,
  3. Seewan Kim1,
  4. Zhuo Chen1,
  5. Geehee Lee1,
  6. Joe Craft2,3,4,5 and
  7. Rong Fan1,4,5
  1. 1Department of Biomedical Engineering
  2. 2Department of Internal Medicine (Rheumatology)
  3. 3Department of Immunobiology
  4. 4Human Translational Immunology Program, Yale University, New Haven, CT 06520, USA
  5. 5Equal contribution from Craft and Fan Laboratories


Background Follicular B helper T (Tfh) cells expand in the circulation of patients with systemic lupus erythematosus and are correlated with pathological outcomes (Choi, et al., Arthritis Rheum. 2015). These cells are both necessary and sufficient to drive pathogenic humoral autoimmunity in murine lupus, and likely in SLE.

Materials and methods We performed multidimensional single cell secretome profiling on Tfh cells from patients with SLE and controls, in parallel with transcriptome (RNA-seq) analysis (including single cell) of Tfh and central memory (Tcm) cells.

Results We find that single Tfh and Tcm cells in SLE are polyfunctional cytokine producers, for example, co-secreting both B-helper IL-21 and inflammation-inducing IFN-γ in excess of that seen in controls. Given their pathogenic potential in lymphoid organs and in disease-affected end organs, such as the lupus kidney, and to dissect the mechanisms underlying the aberrant secretome phenotypes, we performed transcriptome (RNA-seq) analysis (including single cell) of Tfh and central memory (Tcm) cells from SLE patients and healthy donors. Principal component analysis (PCA) of single-cell transcriptomes was done using the top 2000 differentially expressed genes based on ANOVA-testing, revealing four distinct clusters of Tfh and Tcm cells, separating cells from healthy donors and SLE patients. Pathways determined by gene ontology (GO) and pathway enrichment analysis that were highly enriched in SLE T cells included mediators of adaptive responses and inflammation, and those regulating co-stimulation. By contrast, negative regulators of cell proliferation and function were found in the healthy control cluster, and diminished in SLE.

Conclusions Our data demonstrate altered transcriptional programs of lupus Tfh and Tcm cells, and therapeutic targets in disease. They also represent the first detailed transcriptional profiling, and single cell transcriptional profiling, of Tfh cells, the necessary and critical driver of humoral immunity in SLE.

Acknowledgements Supported in part by grants from the NIH/NIAMS (AR40072 and AR053495) and from the Alliance for Lupus Research (to JC).

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.