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319 Association of functional irf7 variants with systemic lupus erythematosus
  1. J Chen,
  2. C Bao,
  3. N Shen and
  4. Q Fu
  1. Renji Hospital- Shanghai Jiaotong University School of Medicine, Rheumatology, Shanghai, China


Background and aims Previous study identified rs1131665 in IRF7 associated with SLE among multiple ethnic groups. This study was undertaken to investigate whether other genetic polymorphisms within KIAA1542/IRF7 confers risk for the development of SLE.

Methods Four SNPs, including rs4963128, rs702966, rs1131665 (Q412R), rs1061502 (K179E) within KIAA1542/IRF7 were genotyped in 784 Chinese SLE patients and 899 controls/IRF7 by using Taqman genotyping assay. Luciferase reporter assay, Co-IP and EMSA were used to assess the effect of K179E polymorphism on the activation of IRF7.

Results Q412R and K179E were significantly associated with SLE in Chinese Han population (p=5.8X10-3, OR=2.33[1.26–4.33], p=2.9X10-3, OR=2.82[1.38–5.76], respectively. IRF7 3’UTR SNP rs702966 was associated with renal involvement (p=0.01 OR=0.46[0.25–0.85]. Compared with expression of IRF7 179E, expression of IRF7 179K risk allele resulted in a 4-fold increase in ISRE transcriptional activity and stronger ISRE binding activity in EMSA (p=0.0002), suggesting IRF7 179K confers elevated IRF7 activity. Further study found 179K (lysine) carrying IRF7 protein showed higher acetylation compared to 179 E (glutamic acid) IRF7.

Conclusions We detected a novel association between rs1061502 (K179E) and SLE susceptibility. K179E could change the acetylation of IRF7 in vitro, which might contribute to the transcriptional activity of IRF7.

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