Article Text

Download PDFPDF

205 Annexin ii-binding immunoglobulin g level correlates with clinical and renal histological disease activity in lupus nephritis
  1. KF Cheung,
  2. S Yung,
  3. M Chau,
  4. DYH Yap and
  5. TM Chan
  1. The University of Hong Kong, Medicine, Pokfulam, Hong Kong S.A.R


Background and aims Annexin II mediates anti-dsDNA antibody binding to mesangial cells and downstream inflammatory and fibrotic processes. We investigated the relationship between annexin II-binding IgG and clinical or histological activity in lupus nephritis.

Methods Serial serum samples from 28 patients with Class III/IV±V lupus nephritis were studied. Annexin II-binding IgG level was measured with an in-house ELISA. Glomeruli were isolated from NZBWF1 mice, gene and protein expression of annexin II and its binding protein p11 were investigated by real-time PCR and cytochemical staining respectively. Ultrastructural localization of annexin II was determined by electron microscopy and immunogold staining.

Results Annexin II-binding IgG level was associated with anti-dsDNA level and disease activity in 42% of lupus nephritis patients. Annexin II-binding IgG level correlated with Activity Index (r=0.44, p=0.04), leukocyte infiltration score (r=0.52, p=0.02), and karyorrhexis/fibrinoid necrosis score (r=0.66, p=0.002) in renal biopsies, and also with the amount of mesangial electron-dense deposit scored semi-quantitatively (r=0.63, p=0.009). Glomerular annexin II and p11 expression increased with disease progression in NZBWF1 mice, and annexin II was found on the surface of mesangial cells and in the mesangial matrix, co-localising with electron-dense deposits.

Conclusions Our data demonstrated an association between annexin II-binding IgG level and clinical/histological disease activity in proliferative lupus nephritis. Co-localization of annexin II with electron-dense deposits suggests a pathogenic role for annexin II.

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.