Abstract
Background Systemic lupus erythematodes (SLE) is an autoimmune disease where T-cell dependent B-cell activation plays a crucial role. Costimulatory marker promote and inhibit immune responses after ligation. Here we analysed the marker BTLA (CD272), PD-1 (CD279), HLA-DR and HVEM on peripheral cyctotoxic T-cells.
Patients and methods 30 SLE-patients fulfilling the American College of Rheumatology (ACR) criteria für SLE and 14 healthy controls (HC) were enrolled. Whole blood staining was performed and samples were analysed by multi-colour flow cytometry. Disease activity was assessed by SLEDAI. Renal involvement was defined as biopsy proven lupus nephritis.
Results The expression of the costimulatory marker PD-1 is signifcantly increased on CD8 +T cells in SLE patients as compared to with healthy controls (31.3%±26.7% vs 15.63%±12.22%). We could not found a significant difference in the expression levels of the BTLA receptor on CD8 +T cells (72,24%±15.33 vs 72.66%±9,4%) in SLE patients as compared to with healthy controls. Also HLA-DR and HVEM was not significantly different expressed on CD8 +T cells in SLE.
Conclusions The costimulytory maker PD-1 is upregualted on cytotoxic T-cells in SLE. Further investigations are needed to elucidate the functional role of this pattern.
