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Genetics, Genomics and Epigenetics
GG-07 Regulatory polymorphisms in EMSY gene are associated with autoantibodies in healthy individuals
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  1. Prithvi Raj1,
  2. Ran Song1,
  3. Benjamin E Wakeland1,
  4. Igor Dozmorov1,
  5. Kasthuribai Viswanathan1,
  6. Chaoying Liang1,
  7. Carlos Arana1,
  8. Bo Zhang1,
  9. JinChun Zhou1,
  10. Bernard R Lauwerys2,
  11. Nancy J Olsen3,
  12. Swapan Nath4,
  13. Peter K Gregersen5,
  14. Judith A James4,
  15. Betty P Tsao6,
  16. Patrick M Gaffney4,
  17. David R Karp7,
  18. Quan-Zhen Li1 and
  19. Edward K Wakeland1
  1. 1Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX, USA
  2. 2Pôle de pathologies, Institut de Recherche Expérimentale et Clinique,Brussels, Belgium
  3. 3Division of Rheumatology, Department of Medicine, Penn State Hershey Medical School, PA, USA
  4. 4Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, OK
  5. 5Feinstein Institute for Medical Research, Manhasset, NY, USA
  6. 6Department of Medicine, Medical University of South Carolina, Charleston, SC, USA
  7. 7Rheumatic Diseases Division, University of Texas Southwestern Medical Center, Dallas, TX, USA

Abstract

Background Systemic lupus erythematosus (SLE) is characterized by the presence of multiple autoantibodies as well as clinical evidence of multi-system immune-mediated pathology. Individuals who develop SLE typically demonstrate serological autoimmunity for several years prior to the onset of clinical disease. Measuring anti-nuclear antibodies (ANA) is a common test for humoral autoimmunity. It is sensitive (95+%) for SLE, but not specific as up to 25% of healthy controls (HC) will have a measurable ANA. While very few of the ANA +HC will develop SLE, they represent a group at higher risk of the disease. Understanding the risks for ANA positivity provides essential knowledge about the development of SLE.

Methods Serum and DNA were collected from 2903 healthy individuals with no personal history of autoimmunity. Antinuclear antibodies were detected using Inova QuantaLite ELISA. Sera from subset (n=724) individuals (ANA− HC, ANA+ HC, and SLE) were assayed by protein microarray quantifying IgM and IgG responses to 89 previously known human autoantigens. A nested cohort consisting of all the ANA +Caucasian individuals and age/gender matched ANA− controls were genotyped using the ImmunoChipv.1 SNP array.

Results In HC, 16% had moderate and 10% had high levels of IgG ANA. Autoantigen microarray data showed that ANA+ HC had a high prevalence of antibodies to non-nuclear and cytoplasmic antigens while subjects with SLE predictably produced antibodies to a variety of nuclear antigens as well. A quantitative genetic association test with ANA identified the locus c11orf30 or EMSY, associated with high ANA phenotype in the healthy population (see figure 1). This locus codes for a negative transcriptional regulator. A haplotype comprised of many potentially regulatory polymorphisms at EMSY contributed to strong risk for ANA in healthy individuals [(p=3.83E-04, OR=2.6)]. eQTL data suggests that the ANA-associated EMSY haplotype leads to reduced expression of EMSY protein in human macrophages and EBV cell lines. Autoantibody profiles of serum samples from healthy individuals with the EMSY risk genotype exhibited high titers of anti-IgG and IgA antibodies targeted to multiple autoantigens as well as food and environmental allergens.

Abstract GG-07 Figure 1
Abstract GG-07 Figure 1

Conclusions EMSY, a locus previously linked with atopy, psoriasis and inflammatory bowel disease was associated with ANA in healthy individuals. The EMSY protein is a BRCA2-associated transcriptional repressor. Individuals with risk haplotypes in EMSY make a wide variety of disease-associated antibodies, suggesting an early common pathway for autoimmune and allergic conditions.

http://dx.doi.org/10.1136/lupus-2018-lsm.94

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