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Original research
Plucked hair follicles from patients with chronic discoid lupus erythematosus show a disease-specific molecular signature
  1. Mohammad Shalbaf1,
  2. Adewonuola A Alase1,
  3. Anna Berekmeri1,2,
  4. Md Yuzaiful Md Yusof1,2,
  5. Jelena Pistolic3,
  6. Mark J Goodfield4,
  7. Sara Edward5,
  8. Natalia V Botchkareva6,
  9. Martin Stacey7,
  10. Edward M Vital1,2 and
  11. Miriam Wittmann1,2
  1. 1Leeds Institue of Rheumatic and Musculoskeletal Medicine, University of Leeds, Leeds, UK
  2. 2National Institute for Health Research (NIHR) Leeds Biomedical Research Centre (BRC), Leeds Teaching Hospitals, Leeds, UK
  3. 3Genomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany
  4. 4Department of Dermatology, Leeds Teaching Hospitals, Leeds, UK
  5. 5Department of Histopathology, Leeds Teaching Hospitals NHS Trust, Leeds, UK
  6. 6Centre for Skin Sciences, Faculty of Life Sciences, University of Bradford, Bradford, UK
  7. 7Faculty of Biological Sciences, School of Molecular and Cellular Biology, University of Leeds, Leeds, UK
  1. Correspondence to Dr Miriam Wittmann; M.Wittmann{at}leeds.ac.uk

Abstract

Objective When faced with clinical symptoms of scarring alopecia—the standard diagnostic pathway involves a scalp biopsy which is an invasive and expensive procedure. This project aimed to assess if plucked hair follicles (HFs) containing living epithelial cells can offer a non-invasive approach to diagnosing inflammatory scalp lesions.

Methods Lesional and non-lesional HFs were extracted from the scalp of patients with chronic discoid lupus erythematosus (CDLE), psoriasis and healthy controls. RNA was isolated from plucked anagen HFs and microarray, as well as quantitative real-time PCR was performed.

Results Here, we report that gene expression analysis of only a small number of HF plucked from lesional areas of the scalp is sufficient to differentiate CDLE from psoriasis lesions or healthy HF. The expression profile from CDLE HFs coincides with published profiles of CDLE from skin biopsy. Genes that were highly expressed in lesional CDLE corresponded to well-known histopathological diagnostic features of CDLE and included those related to apoptotic cell death, the interferon signature, complement components and CD8+ T-cell immune responses.

Conclusions We therefore propose that information obtained from this non-invasive approach are sufficient to diagnose scalp lupus erythematosus. Once validated in routine clinical settings and compared with other scarring alopecias, this rapid and non-invasive approach will have great potential for paving the way for future diagnosis of inflammatory scalp lesions.

  • cutaneous lupus
  • scarring alopecia
  • hair follicle
  • diagnostic
  • interferon-stimulated genes

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Footnotes

  • Contributors Study design: MW, MJG, EMV; Clinical contribution: AB, MW, MJG, EMV, MYMY; Laboratory-based experiments: MSh, AAA, JP; Data analysis: MSh, MW, MSt, EMV; Contribution to critical discussion of results: MSt, NVB, MJG, SE, EMV, MW; Manuscript writing and revision: MSh, AAA, SE, MJG, MYMY, AB, NVB, MSt, EMV, MW.

  • Funding This project was mainly supported by a grant from Lupus UK with additional funding by Medical Research Council grant MR/M01942X/1. MYMY is funded as an National Institute for Health Research (NIHR) Doctoral Research Fellow and EMV is funded as an NIHR clinician scientist. This research is also supported by the NIHR Leeds Biomedical Research Centre.

  • Disclaimer The views expressed are those of the author(s) and not necessarily those of the National Health Service, the NIHR or the Department of Health.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement Data are available in a public, open access repository. All data relevant to the study are included in the article or uploaded as supplementary information.

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