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Abstracts
261 MiR-326 regulates CD4+T cells differentiation in lupus disease of MRL/lpr mice
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  1. Yuan Xia1,
  2. Xuan Fang1,
  3. Nan Xiang1,
  4. Xiaojuan Dai1,
  5. Li Jin1,
  6. Xiaomei Li1,
  7. Jinhui Tao2 and
  8. Xiangpei Li3
  1. 1Department of Rheumatology and Immunology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China
  2. 2Department of Rheumatology and Immunology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui
  3. 3Department of Rheumatology and Immunology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei

Abstract

Background CD4 +T cells play a major role in systemic lupus erythematous (SLE). Many aberrations in miR-326 expression have been described as related to abnormal T cell activation in SLE. The aim of this study was to investigate the effect of miR-326 expression on the differentiation of CD4 +T cells in MRL/lpr mice.

Methods 3 groups of female MRL/lpr mice were injected with lentivirus-miR-326 (LV-326) or lentivirus-miR-326 specific inhibitor (LV-sponge) to increase or inhibit miR-326 expression, respectively, and lentivirus-no-encoding (LV-ctrl) as control10 mice per group. The percentage of Th17, Th1, and Treg cells in spleen were determined by flow cytometry, the expression levels of CD4 +T related cytokines were determined by CBA and ELISA.

Results The results showed that, compared with LV-ctrl mice and LV-sponge mice, LV-326 mice had higher percentage of Th17 cells, and lower percentage of Tregs and Th1 cells in splenic CD4 +T cells. In contrast, LV-sponge mice had lower percentage of Th17 cells as well as higher percentage of Tregs and Th1 cells than LV-ctrl mice in splenic CD4 +T cells. Moreover, serum levels of IL-17A were significantly increased in LV-326 mice, compared with LV-ctrl mice and LV-sponge mice. Serum levels of IL-2 and TGF- were decreased in LV-326 mice compared with LV-ctrl mice.

Conclusions These findings suggesting that miR-326 regulates CD4 +T cells differentiation and inflammatory-related cytokines production in lupus model mouse. Implying that miR-326 may play a vital role in SLE pathogenesis by regulating CD4 +T cells differentiation.

Funding Source(s): the National Natural Science Foundation of China (81373186)

http://dx.doi.org/10.1136/lupus-2019-lsm.261

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