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P97 Deficiency of marginal-zone B cells in peripheral blood of SLE patients in clinical remission or low disease activity state in a long-term study
  1. Zbynek Hrncir1,
  2. Doris Vokurkova2,
  3. Marcela Drahosova2 and
  4. Tomas Soukup1
  1. 12nd Dept. of Internal Medicine, University Hospital, Hradec Králové
  2. 2Dept. of Immunology and Allergy, University Hospital, Hradec Králové, Czech Republic


Purpose Deficiency of marginal-zone B cells was observed in peripheral blood (PB) of SLE pts in clinical remission or low disease activity (LDA)1. Goal of the prospective, comparative, long-term study is follow-up of this phenomenon.

Methods Forty five adult SLE (ACR/1982, updated 1997) pts in complete remission or LDA2 and 10 age- and sex-matched healthy controls (HC) were enrolled in „month 0’, and SLE also after twelve-months („month 12’) and 36-months („month 36’) period; overlap syndromes, infection, renal failure and monoclonal gammopathy in SLE were excluded. The DuraClone IM panel (Beckman Coulter) was used to identify CD19+CD27+IgM+ B cell subpopulation in PB samples by flow cytometry navios (Beckman Coulter) with software analysis using Kaluza version 1.2.; data obtained were expressed in relative% of PB lymphocytes and absolute values x106/L, and processed using Medcalc-Statistical Software programme.

Results Significant differences (p =0.002 - <0.001) were obtained between absolute values of CD19+CD27+IgM+ B cells in HC (median 31.36, 95% CI 21.49 – 63.35) and SLE „month 0’ (median 13.17, 95% CI 7.87 – 17.09), SLE „month 12’ (median 10.56, 95% CI 7.24 – 16.04), and SLE „month 36’ (median 9.66, 95% CI 7.22 – 13.21), but not between values obtained in SLE „month 0’, „month 12’ and „month 36’ (p>0.05); not significant differences were found using analysis according to relative% of B cells under study (p>0.05).

Conclusion Data obtained demonstrated a long-term deficiency of marginal-zone B cells in PB of SLE pts in complete remission or LDA; susceptibility to infection should be supposed, but further studies are necessary.

Acknowledgement Research project PROGRES Q40-15, Charles Univ. Faculty of Medicine, Hradec Králové, Czech Republic.


  1. Hrncir Z, et al. Clin Exper Rheumatol2016;34(S99);S–63.

  2. Fanouriakis A, et al. Ann Rheum Dis 2019;78;736–745.

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