Quinolinic acid, a kynurenine/tryptophan pathway metabolite, associates with impaired cognitive test performance in systemic lupus erythematosus
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* Erik W Anderson
* Joanna Fishbein
* Joseph Hong
* Julien Roeser
* Richard A Furie
* Cynthia Aranow
* Bruce T Volpe
* Betty Diamond
* Meggan Mackay

## Abstract

**Objectives** Interferon-alpha, an important contributor to SLE pathogenesis, induces the enzyme indoleamine 2,3-dioxygenase in the kynurenine/tryptophan (KYN/TRP) pathway. This leads to a potentially neurotoxic imbalance in the KYN/TRP pathway metabolites, quinolinic acid (QA), an N-methyl D-aspartate glutamatergic receptor (NMDAR) agonist, and kynurenic acid (KA), an NMDAR antagonist. We determined whether QA/KA ratios associate with cognitive dysfunction (CD) and depression in SLE.

**Methods** This cross-sectional study included 74 subjects with SLE and 74 healthy control (HC) subjects; all without history of neuropsychiatric disorders. Serum metabolite levels (KYN, TRP, QA, KA) were measured concurrently with assessments of cognition (Automated Neuropsychological Assessment Metrics (ANAM), 2×2 array), mood and pain, and compared between SLE and HC. Multivariable modelling in SLE was used to evaluate associations of metabolites with cognitive performance and depression.

**Results** Serum KYN/TRP and QA/KA ratios were elevated in SLE versus HC (p<0.0001). SLE performed worse than HC on four of five ANAM tests (all p≤0.02) and the 2×2 array (p<0.01), and had higher depression scores (p<0.01). In SLE, elevated QA/KA ratios correlated with poor performance on Match to Sample (MTS), a working memory and visuospatial processing task (p<0.05). Subjects with SLE with elevated QA/KA ratios also had slightly higher odds of depression, but this did not reach significance (p=0.09). Multivariable modelling in SLE confirmed an association between QA/KA ratios and poor MTS performance when considering potentially confounding factors (p<0.05).

**Conclusions** Elevated serum KYN/TRP and QA/KA ratios confirm KYN/TRP pathway activation in SLE. The novel association between increased QA/KA ratios and poor cognitive performance supports further study of this pathway as a potential biomarker or therapeutic target for SLE-mediated CD.

*   lupus erythematosus
*   systemic
*   autoimmunity
*   cytokines
*   inflammation

### Key messages

#### What is already known about this subject?

*   Interferon-alpha contributes to lupus pathogenesis and induces the enzyme indoleamine 2,3-dioxygenase in the kynurenine/tryptophan (KYN/TRP) pathway, which leads to a potentially neurotoxic imbalance of elevated quinolinic acid (QA) relative to kynurenic acid (KA).

*   An elevated QA/KA ratio associates with cognitive dysfunction in neurodegenerative diseases and idiopathic depression.

#### What does this study add?

*   It was not known whether elevated QA/KA ratios were present in lupus, and whether they associated with cognitive dysfunction or depression in patients with lupus. In this study, elevated QA/KA ratios in patients with lupus associate with cognitive dysfunction; namely, poor performance in working memory and visuospatial processing.

#### How might this impact on clinical practice or future developments?

*   The association of elevated QA/KA ratios with cognitive dysfunction in patients with lupus, supported by a plausible pathophysiological mechanism of interferon-alpha stimulation of the KYN/TRP pathway, suggests a potential therapeutic target that deserves future study.

## Introduction

Cognitive dysfunction (CD) is an insidious, highly prevalent and often devastating manifestation of SLE with significant impact on quality of life.1 Abnormal brain imaging and evidence of CD in patients with new-onset disease suggest that SLE-related mechanisms contribute to CD irrespective of other potentially neurotoxic processes such as infection, comorbid illness and medications.2 3 Several mechanisms for SLE-mediated CD (SLE-CD) have been identified in animal models, including pathways mediated by autoantibodies, cytokines and activated microglia.1 Metabolites of the kynurenine/tryptophan (KYN/TRP) pathway, quinolinic acid (QA) and kynurenic acid (KA), modulate neuronal function through N-methyl-D-aspartate (NMDA) glutamatergic receptors (NMDARs) and are implicated in the pathogenesis of CD in other neurodegenerative diseases, idiopathic depression and suicidal ideation, and depression in interferon-alpha (IFNα)-treated patients with hepatitis C.4–8

The KYN/TRP pathway is catalysed by the enzyme indoleamine 2,3-dioxygenase (IDO) present in immune cells in the periphery and brain.6 IFNα, an important contributor to SLE pathogenesis, induces IDO,9 which catalyses the breakdown of TRP into KYN that is further metabolised to either QA or KA (figure 1). NMDARs on neurons are integral to learning and memory.10 As an NMDAR agonist, QA can cause glutamatergic excitotoxicity, while KA is an NMDAR antagonist that can protect neurons from excitotoxic damage.6 Animal studies demonstrate an association between peripheral IFNα and microglia activation, and a negative association between microglial QA and neurite outgrowth and complexity.11 12 Inhibitors of IDO or NMDAR prevented QA-provoked neurotoxicity in an animal model,12 suggesting that an elevated QA/KA ratio may drive NMDAR-related neurotoxicity, affecting cognitive function.

![Figure 1](http://lupus.bmj.com/https://lupus.bmj.com/content/lupusscimed/8/1/e000559/F1.medium.gif)

[Figure 1](http://lupus.bmj.com/content/8/1/e000559/F1)

Figure 1 
The kynurenine/tryptophan (KYN/TRP) pathway. This is a simplified schematic of the KYN/TRP pathway, highlighting the intermediates and enzymes involved in the production of quinolinic acid (QA) and kynurenic acid (KA). The enzyme IDO is stimulated by inflammatory cytokines, such as IFN⍺, that results in the breakdown of TRP into KYN. KYN may be further metabolised by KMO ultimately to QA, an NMDAR agonist, or by KAT to KA, an NMDAR antagonist. Since the enzyme KMO has higher affinity for KYN than KAT, metabolism proceeds preferentially towards the production of QA in the setting of inflammation.55 IDO, indoleamine 2,3-dioxygenase; IFNα, interferon-alpha; IFNγ, interferon-gamma; NMDAR, N-methyl D-aspartate glutamatergic receptor; TNFα, tumor necrosis factor-alpha.

Elevated serum KYN/TRP ratios, reflective of IDO activity, are reported in subjects with SLE9 13 14 and associated with an IFN signature.9 Metabolomic studies of peripheral blood mononuclear cells in human SLE identified elevated KYN levels as a contributor to mammalian target of rapamycin (mTOR) activation,15 associated with T cell dysfunction in SLE.16 Treatment with N-acetylcysteine in a small SLE clinical trial resulted in improved self-report of cognitive abilities,17 decreased KYN levels15 and decreased mTOR activity in T cells.18 Elevated cerebrospinal fluid (CSF) QA levels are reported in neuropsychiatric SLE (not limited to CD) compared with those with central nervous system (CNS) dysfunction unrelated to SLE or healthy control (HC).19 While these studies suggest altered KYN/TRP pathway metabolism in SLE, perhaps driven by IFNα-induced IDO, associations between TRP metabolites and CD and depression in SLE have not been directly studied.

We hypothesised that KYN/TRP pathway activation in SLE, perhaps mediated by IFNα, contributes to SLE-CD and depression by promoting a QA/KA imbalance. We measured serum levels of KYN, TRP, KA and QA in a cohort of subjects with SLE and HC, and concurrently assessed cognitive performance and depression.

## Patients and methods

### Subject selection

Seventy-four female subjects with SLE were recruited from Northwell Health Rheumatology Clinics. All were aged ≥18 years and fulfilled SLE criteria.20 21 To avoid confounding influences on neuropsychological testing, key exclusion criteria for subjects with SLE included any history of neurological disease, psychiatric illness requiring medication that preceded SLE diagnosis, alcohol abuse or illicit drug use, acute infection, and current narcotic or psychiatric medication use. Patients with psychiatric illnesses diagnosed after SLE onset were allowed as long as they were not currently taking antidepressant or antipsychotic medications. Seventy-four female HC subjects, comparable with SLE subjects in age, ethnicity and race, were recruited. The HC subjects had no history of chronic medical or psychiatric illnesses and were on no medications other than oral contraception.

### Patient and public involvement

Patients were not directly involved in the design, recruitment or conduct of the study.

### Clinical, neuropsychological and behavioural assessments

All clinical and neuropsychological assessments, and metabolite measurements were completed within a 10-day period. For all subjects, cognition was assessed using the Automated Neuropsychological Assessment Metrics (ANAM)22 computerised test battery (selected tests and associated cognitive domains detailed in table 2) and a 2×2 array to assess object recognition and spatial memory.23 Throughput scores (accuracy÷reaction time) were the primary measures of cognitive efficiency. Depression was evaluated using the Beck Depression Inventory (BDI; scores range from 0 to 63, scores >13 indicate clinically significant depression). Additional assessments included the Numerical Rating Scale (NRS) for pain (0–10; ‘0’ represents no pain, ‘10’ represents worst pain imaginable) and ANAM mood subtests for fatigue, sleepiness, anger, happiness, restlessness, vigour and anxiety. For subjects with SLE, disease activity was assessed with the SELENA-SLEDAI24 and Physician’s Global Assessment (PGA).25 Accrued damage since SLE diagnosis was measured with the SLICC Damage Index (SLICC-DI).26 Testing was administered by authors EWA or JH in a quiet room to minimise distractions.

### Analysis of KYN/TRP pathway metabolites (KYN, KA, TRP and QA)

Metabolite concentrations in de-identified, blinded serum samples were measured by Charles River Laboratories ([www.criver.com](http://www.criver.com)) using high-performance liquid chromatography with tandem mass spectrometry (see online supplemental material).

### Supplementary data

[[lupus-2021-000559supp001.pdf]](pending:yes)

### Statistical analyses

Metabolite data and select cognitive outcome measures (all but Running Memory Continuous Performance Test (RMCPT) and Non-Spatial Memory (NSM); see table 2) were log-transformed to better meet normality assumptions. Differences between SLE and HC for demographic and clinical characteristics, neuropsychological and behavioural test scores, and KYN/TRP pathway metabolites were assessed using t-tests, Mann-Whitney U tests or Χ2 tests, as appropriate.

Associations between demographic and clinical variables, metabolite levels and cognitive performance were analysed with Spearman’s rank-order correlation, Mann-Whitney U test, Kruskal-Wallis test, Χ2 test or Fisher’s exact test, as appropriate. Among subjects with SLE, multivariable linear regression modelling was performed to evaluate associations between individual neuropsychological test performance and clinical variables and metabolite levels. Multivariable modelling for the outcome of depression was also performed in subjects with SLE using ordinal multinomial regression methods whereby BDI was assessed as a 3-level outcome (none (BDI=0), minimal (>0–≤13) and clinically significant depression (>13)).

The following variables were considered for inclusion in the multivariable models: age; ethnicity; race (black, white, other); computer experience; education years; tobacco use (ever); marijuana use (ever); history of hypertension; psychiatric illness after SLE diagnosis requiring medication prior to recruitment for this study; history of peripheral thrombosis; white blood cell count (WBC; as a continuous variable and categorised as <3.0 x 109/L or ≥3.0 x 109/L); haemoglobin; platelet count; BDI (categorised as 0; >0–≤13; >13 or ≤13; >13) and NRS scores; ANAM mood subtest scores for fatigue, sleepiness, anger, anxiety, restlessness, happiness and vigour; serum levels of QA, KA, TRP, KYN, QA/KA and KYN/TRP; disease duration; history of lupus nephritis; history of SLE-related psychosis; prednisone dose; immunosuppressant and hydroxychloroquine use; SLEDAI and clinical SLEDAI (all descriptors except anti-double-stranded DNA (anti-dsDNA) antibodies and complement) scores; PGA; SLICC-DI; anti-dsDNA; C3, C4; urinary protein/creatinine ratio; blood urea nitrogen; creatinine; anti-Ro and anti-La (±).

Variables on univariate screen with p<0.1 were considered for inclusion in each multivariable regression model. Due to skewness or lack of variability in the data, some variables were log-transformed (WBC and platelet counts) or categorised (WBC counts, BDI scores, NRS scores, all ANAM mood subtest scores), as appropriate. Multicollinearity was assessed and addressed, as appropriate. Models were created using a backward elimination strategy. The final model was chosen based on the lowest Akaike Information Criterion (AIC). If competing with a more parsimonious model, and the decrease in AIC was marginal, the more parsimonious model was selected. Analyses were conducted using SAS V.9.4 (SAS Institute). All tests of significance were two-sided, and the significance level was set at alpha=0.05.

## Results

### Subject characteristics

Few significant differences were identified between subjects with SLE and HC subjects (table 1). Compared with HC subjects, a higher percentage of subjects with SLE were unemployed, had a self-reported history of CD and had less computer experience (table 1). In addition, a higher percentage of subjects with SLE had low WBC and haemoglobin levels, and high platelet counts. Scores on the SLEDAI ranged from 0 to 29 and current prednisone doses ranged from 0 to 75 mg daily (table 1), indicating a broad range of disease activity.

View this table:
[Table 1](http://lupus.bmj.com/content/8/1/e000559/T1)

Table 1 
Demographics and characteristics of subjects with SLE and healthy controls (HC)

### Cognitive and behavioural testing in SLE compared with HC

Compared with HC, subjects with SLE performed significantly worse on four of five ANAM cognitive tests and the 2×2 array (table 2, p<0.03). Most subjects with SLE scored below the HC mean for each test: 89% scored below the HC mean in RMCPT, 84% scored below in Match to Sample (MTS), 78% in Spatial Memory Test (SM), 77% in NSM, 70% in Matching Grids (MG) and 57% in Simple Reaction Time. Poor task performance (measured by throughput scores: accuracy÷reaction time) was due to both worse accuracy and slower reaction time in MTS and NSM, while it was due to slower reaction time (with comparable accuracy) in MG, RMCPT and SM. Subjects with SLE also had higher scores for depression, pain, fatigue, sleepiness, anger and anxiety (table 2, p<0.01). Among the subjects with SLE, cognitive task performance and depression were not affected by employment status (data not shown).

View this table:
[Table 2](http://lupus.bmj.com/content/8/1/e000559/T2)

Table 2 
Neuropsychological and behavioural test performance in subjects with SLE compared with healthy controls (HC)

### KYN/TRP pathway metabolites and associations with cognitive testing, depression

Subjects with SLE had higher KYN/TRP ratios (0.043 µM (0.033–0.060); median (Q1–Q3)) compared with HC (0.032 (0.025–0.037)) (p<0.0001). Similarly, higher QA/KA ratios were found in SLE (18.3 nM (10.5–25.2)) compared with HC (9.0 nM (6.2–12.2)) (p<0.0001) (figure 2). Among subjects with SLE, elevated QA/KA ratios correlated with poor performance on MTS (r=−0.24, p=0.04). Subjects with SLE with elevated QA/KA ratios also had slightly higher odds of depression, but this did not reach significance (OR=1.0, 95% CI=1.0 to 1.1, p=0.09). Among HC, QA/KA ratios did not correlate with performance on any cognitive test.

![Figure 2](http://lupus.bmj.com/https://lupus.bmj.com/content/lupusscimed/8/1/e000559/F2.medium.gif)

[Figure 2](http://lupus.bmj.com/content/8/1/e000559/F2)

Figure 2 
KYN/TRP pathway metabolite ratios in subjects with SLE compared with healthy controls (HC). Serum metabolite ratios measured by HPLC-MS in 74 subjects with SLE and 74 HC. Subjects with SLE demonstrate a significantly higher KYN/TRP ratio (A) and QA/KA ratio (B) than HC. HPLC-MS, high-performance liquid chromatography-mass spectrometry; KA, kynurenic acid; KYN, kynurenine; QA, quinolinic acid; TRP, tryptophan.

### Associations between SLE disease characteristics and metabolite ratios, cognitive and behavioural testing

To ascertain potential confounding effects, associations between SLE-related variables (disease activity, duration, medication use) and metabolite levels, cognitive performance and behaviour were assessed. Since increased disease activity and active serology associates with increased IFNα activity,27 and potentially KYN/TRP pathway activation, we assessed their relationship with metabolite ratios. Elevated KYN/TRP ratios did not associate with disease activity assessed with SLEDAI (r=0.00, p=0.98) or PGA (r=0.03, p=0.80). A positive correlation was identified between QA/KA ratios and SLEDAI scores (r=0.24, p=0.04); however, no correlations were found between QA/KA ratios and clinical SLEDAI, exclusive of serology (r=0.12, p=0.30) or PGA scores (r=0.14, p=0.23). Significantly higher QA/KA ratios were found in those with elevated anti-dsDNA antibodies and hypocomplementemia (low C3 and/or low C4) (N=33, mean±SD=26.2±20.8 nM) compared with those without this serological profile (N=41, 16.0±8.7 nM) (p<0.01).

No associations between disease activity and cognitive performance were identified except for modest positive associations between MTS scores and SLEDAI (r=0.24, p=0.04) and PGA (r=0.25, p=0.03) scores. Cognitive performance also did not associate with prednisone dose, immunosuppressant use or depression scores, but did associate with disease duration. Subjects with SLE with disease duration ≥10 years (N=42, mean±SD=18.0±6.7 years) compared with those with duration <10 years (N=32, 4.8±2.4 years) demonstrated significantly worse MTS scores (23.5±7.3 vs 28.0±9.6, p=0.04). There were no differences in KYN/TRP or QA/KA ratios between subjects with SLE with disease duration ≥10 years and those <10 years.

Significant positive associations were found between disease activity and depression scores (SLEDAI (r=0.40, p<0.01) and PGA (r=0.41, p<0.01)), pain (SLEDAI (r=0.34, p=<0.01) and PGA (r=0.36, p<0.01)), sleepiness (SLEDAI (r=0.25, p=0.04) and PGA (r=0.26, p=0.03)), fatigue (SLEDAI (r=0.24, p=0.04) and PGA (r=0.27, p=0.02)) and anxiety (SLEDAI (r=0.35, p<0.01) and PGA (r=0.38, p<0.01)). There was no association between anger scores and disease activity.

### Multivariable modelling for cognitive dysfunction and depression

Multivariable model building included the following variables that associated with poor cognitive performance in subjects with SLE (table 3): increased age, history of psychiatric illness after SLE diagnosis requiring medication prior to the current study, increased platelet count, history of peripheral thrombosis, history of hypertension, anxiety, decreased computer experience and an elevated QA/KA ratio. While clinically significant depression was more likely in subjects with higher disease activity and an elevated QA/KA ratio, the final, best-fit model for predicting depression included anxiety (associated with worse depression scores, p<0.01) and happiness (associated with better scores, p<0.05).

View this table:
[Table 3](http://lupus.bmj.com/content/8/1/e000559/T3)

Table 3 
Results of multivariable models to determine predictors of neuropsychological test performance in subjects with SLE

## Discussion

This is the first report of an association between an imbalanced ratio of the KYN/TRP pathway metabolites, QA/KA, and poor performance on a task of working memory and visuospatial processing in SLE, suggesting a potential role for this pathway in SLE-CD. In this cohort, subjects with SLE demonstrated impaired cognitive performance compared with HC, unrelated to potentially confounding influences from disease activity, antiphospholipid syndrome (APS), medication use, educational level, employment status, fatigue, sleepiness and pain. Subjects with SLE demonstrated elevated serum KYN/TRP ratios, suggesting IDO activation consistent with prior studies,9 13 14 and elevated QA/KA.

Increased QA/KA ratios selectively associated with poor MTS task performance. The MTS test requires a subject to select which of two checkerboard matrix designs matches the one presented 5 s earlier and is a test of working memory and visuospatial processing. Poor MTS performance was unique among the other ANAM tasks in that it was due to both poor accuracy and slower reaction time, rather than slower reaction time alone, perhaps representing more severe impairment. Overall, subjects with SLE performed worse than HC on every task involving working memory, supporting findings by our group and others of poor working memory performance in SLE.23 28 These data suggest that elevated QA/KA ratios may contribute to a pathological mechanism for SLE-mediated working memory impairment. Moreover, elevated QA/KA ratios may be a therapeutic target, which is indirectly supported by a study demonstrating improved cognition in patients with SLE treated with N-acetylcysteine,17 which prevented KYN accumulation.15

The prefrontal cortex (PFC) governs aspects of working memory and visuospatial processing29 30 and is part of a functional network engaged during MTS task performance.31 We previously demonstrated correlations between increased disease duration and diminished PFC activation on functional MRI and impaired performance on a working memory task.2 Similarly, we demonstrated decreased PFC metabolism in subjects with SLE that correlated with longer disease duration23 32 and decreased PFC volume is associated with elevated QA/KA ratios in depressed subjects without SLE.7 Combined with our findings of associations among elevated QA/KA, poor working memory task performance and longer disease duration, these studies suggest a potential role for accumulated exposure to elevated QA/KA levels in PFC damage and working memory deficits. In the absence of a longitudinal study, this is a cautious interpretation as age and other factors may be important confounders.

Potential biomarkers for SLE-CD include serum levels of antibodies to NMDAR, ribosomal P protein/neuronal surface P antigen and phospholipid.1 33 Despite specific pathogenic mechanisms that contribute to neuron death or functional impairment, and associations with CD in animal models, they have not proven to be sensitive or specific for human SLE-CD.1 33 Advanced neuroimaging techniques are beginning to demonstrate abnormal structural or functional patterns that associate with SLE-CD.34 The potential contribution of KYN/TRP pathway activation and an elevated QA/KA ratio to SLE-CD is important because this pathway may represent a modifiable therapeutic target.

Disease activity is an important potential confounder in measurement of cognitive ability and associates with CD in some studies, while not in others.1 In our cohort, increased disease activity did not associate with poor cognitive performance. In contrast, a modest positive correlation was found between SLEDAI scores and MTS performance, independent of medication effects. However, anti-dsDNA antibodies and hypocomplementemia associated with elevated QA/KA ratios. This is relevant in light of our hypothesis that IDO stimulation by IFNα contributes to increased TRP metabolism and a QA/KA imbalance, as active serology associates with high IFNα activity in SLE.27 Further direct study of the relationship between IFNα and the pathway is underway. Another potential confounder in measurement of cognitive ability is APS, mediated by potential effects on CNS microvasculature. However, only one subject in this cohort had a history of APS with no known CNS disturbance, and only 5 of 74 subjects (7%) had elevated anticardiolipin IgG, IgM or IgA antibodies. These subjects did not perform significantly worse on any cognitive test (data not shown) than the 69 subjects who were negative for anticardiolipin IgG, IgM and IgA antibodies.

The multivariate analyses yielded increased platelets and a history of peripheral thrombosis as additional unexpected predictors of poor cognitive performance in SLE. Thrombocytosis, with35 36 or without37 thrombosis, has been associated with CD in myeloproliferative disorders and chronic inflammatory diseases, including SLE and other neurodegenerative conditions.36 38 39 Our study excluded subjects with known CNS vascular disease; however, standard neuroimaging may fail to detect microinfarction.40 41 Platelet activation is a contributor to cardiovascular, renal and thrombotic disease in SLE due to proinflammatory mediators such as type I IFN.36 Therefore, our observation that increased platelets associated with poor cognitive performance deserves further study.

Depression is highly prevalent in SLE but attribution to SLE-mediated mechanisms apart from other factors is compromised by limited studies of biological mechanisms in humans. In patients without SLE, elevated serum QA/KA associates with severe depression requiring electroconvulsive therapy42 43 and elevated CSF QA levels were found in suicidal patients and in brains of suicide completers.7 Notably, only three subjects with SLE in our cohort had severe depression (BDI score >28). These subjects had higher mean QA/KA ratios (N=3, mean±SD=29.2±9.5) than subjects without severe depression (BDI ≤28, N=71, 20.2±16.2), but this did not reach significance (p<0.1). The lack of a significant association between QA/KA ratios and depression may be related to the small number of severely depressed subjects with SLE in our cohort.

There are several potential limitations to this study. First, in the absence of preliminary data on associations between metabolite ratios and cognitive performance or depression, the study was powered to detect differences in metabolite ratios between SLE and HC. It is possible that elevated QA/KA ratios may associate with poor performance on additional tests or depression in a larger cohort. Second, CSF metabolite levels would provide direct evidence of KYN/TRP metabolites in the brain. KYN and TRP cross the blood–brain barrier more easily than QA and KA;44 however, both peripheral KYN and QA contribute to brain levels of these metabolites under systemic inflammatory conditions,45 and several human studies report positive correlations between blood and CSF levels of KYN, TRP46–48 and QA.49–51 Our study was cross-sectional, and chronic KYN/TRP pathway activation may negatively impact SLE-CD longitudinally, suggested by associations among disease duration, working memory and QA/KA levels. Studies are underway to determine longitudinal changes in KYN/TRP pathway metabolites and associations between IFNα and metabolite ratios and changes in cognition and depression. Future studies may also consider comparator groups consisting of primary neuropsychiatric or other autoimmune diseases. While we attempted to control for drugs that affect IDO, exclusion of all potentially interfering drugs, such as glucocorticoids, would have negatively impacted the study’s feasibility and generalisability. However, most of these drugs inhibit rather than stimulate IDO,52 53 which would have reduced our chance of identifying elevated metabolite ratios in SLE. Lastly, dietary TRP intake was not assessed, although dietary impact on KYN/TRP pathway metabolism is uncertain.54

Our carefully conducted assessments of subjects with SLE without confounding CNS disease identified a novel association between elevated QA/KA ratios and poor cognitive performance. The established neurotoxicity of QA, coupled with a plausible pathophysiological mechanism for a QA/KA imbalance in SLE by IFNα stimulation of IDO, support additional study of the KYN/TRP pathway as a potential biomarker or therapeutic target for SLE-CD. The therapeutic potential of the pathway is highlighted by the development of non-immunosuppressive KA analogues or KYN/TRP pathway enzyme inhibitors8 to shift QA/KA ratios towards a neuroprotective balance.

## Data availability statement

All data relevant to the study are included in the article or uploaded as supplemental information.

## Ethics statements

### Patient consent for publication

Not required.

### Ethics approval

The research complied with the Helsinki Declaration and was approved by Northwell Health’s Institutional Review Board. Informed consent was obtained prior to study procedures.

## Acknowledgments

The authors would like to express their gratitude towards the patients and healthy individuals who participated in this study, and for the support of the research coordinators and nurses at the Feinstein Institutes.

## Footnotes

*   Presented at This work was previously presented as a poster at the American College of Rheumatology Convergence 2020 (Anderson E Fishbein J, Hong J, *et al*. Impact of the Kynurenine/Tryptophan Pathway on Cognitive Dysfunction and Depression in Systemic Lupus Erythematosus (abstract). Arthritis Rheumatol. 2020;72(suppl10)).

*   Contributors Study design—EWA, MM, CA and BD. Data collection—EWA, MM, CA, RAF, JR and JH. Data analysis—EWA, JF, MM, CA, BD, BV and JR. Interpretation of findings—EWA, JF, MM, CA, BD, BV and RAF. Preparation of manuscript—EWA, JF, MM, CA, BD, BV, RAF and JR. Guarantor—EWA. All authors read and approved the final manuscript.

*   Funding This work was supported by a grant from the Lupus Foundation of America (Gary S Gilkeson Career Development Award, G-1904-00773, PI: EWA; October 2019–September 2021).

*   Competing interests None declared.

*   Provenance and peer review Not commissioned; externally peer reviewed.

*   Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise.

*   Received August 12, 2021.
*   Accepted October 12, 2021.


*   © Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

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