Article Text
Abstract
Objective Recent studies reported that SLE is characterised by altered interactions between the microbiome and immune system. We performed a meta-analysis of publications on this topic.
Methods Case–control studies that compared patients with SLE and healthy controls (HCs) and determined the diversity of the gut microbiota and the abundance of different microbes were examined. Stata/MP V.16 was used for the meta-analysis. A Bonferroni correction for multiple tests was used to reduce the likelihood of false-positive results.
Results We included 11 case–control studies that examined 373 patients with SLE and 1288 HCs. These studies were performed in five countries and nine cities. Compared with HCs, patients with SLE had gut microbiota with lower Shannon-Wiener diversity index (weighted mean difference=−0.22, 95% CI −0.32 to –0.13, p<0.001) and lower Chao1 richness (standardised mean difference (SMD)=−0.62, 95% CI −1.04 to –0.21, p=0.003). Patients with SLE had lower abundance of Ruminococcaceae (SMD = −0.49, 95% CI −0.84 to −0.15,p=0.005), but greater abundance of Enterobacteriaceae (SMD=0.45, 95% CI 0.01 to 0.89, p=0.045) and Enterococcaceae (SMD=0.53, 95% CI 0.05 to 1.01, p=0.03). However, only the results for Ruminococcaceae passed the Bonferroni correction (p=0.0071). The two groups had no significant differences in Lachnospiraceae and Bacteroides (both p>0.05). Patients with SLE who used high doses of glucocorticoids had altered gut microbiota based on the Chao1 species diversity estimator, and hydroxychloroquine use appeared to reduce the abundance of Enterobacteriaceae.
Conclusions Patients with SLE have imbalanced gut microbiota, with a decrease in beneficial bacteria and an increase in harmful bacteria. Drugs used to treat SLE may also alter the gut microbiota of these patients.
- systemic lupus erythematosus
- autoimmune diseases
- autoimmunity
- outcome assessment, health care
Data availability statement
Data are available upon reasonable request.
This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.
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Key messages
What is already known about this subject?
SLE is characterised by altered interactions between the microbiome and immune system.
What does this study add?
Ruminococcaceae, Enterococcaceae and Enterobacteriaceae were imbalanced in patients with SLE.
The dosage of glucocorticoids and use of hydroxychloroquine alter the abundance of some gut microbes.
How might this impact on clinical practice or future developments?
This study proposes some factors that may cause discrepant results in the gut microbiota in SLE and provides references for future research.
Introduction
SLE is a chronic and systemic autoimmune disease that has complex clinical manifestations and is characterised by excessive activation of B cells and T cells, production of autoantibodies, and injury of multiple organs and tissues.1 Although there have been improvements in diagnosis and treatment strategies, treatment efficacy remains inadequate due to limited understanding of the aetiology and pathogenesis of SLE. The morbidity and mortality of SLE therefore continue to increase.2 3
Recent studies found that the gut microbiota has a role in the development and regulation of the immune system.4 5 In particular, disruption of the gut microbiota may affect autoimmunity by altering the balance between tolerogenic and inflammatory microbes.6 Therefore, studies on alterations in the gut microbiota of patients with SLE may provide a new avenue for examining the pathogenesis of SLE.
Researchers initially discovered the presence of dysbiosis in the gut microbiota of patients with SLE, in that patients with SLE had a lower ratio of Firmicutes to Bacteroidetes than healthy controls (HCs).7 8 This led to speculation on the underlying connection between SLE and intestinal microbes. One hypothesis is that a disrupted gut barrier leads to a ‘leaky gut’, which allows bacteria to transfer to other tissues, thus activating immune factors and causing systemic autoimmunity.9 The decrease in gut microbial diversity and diminution of Lactobacillaceae may function in this response.10 Another hypothesis is that stimulation of specific molecular pathways by the gut microbiota induces SLE. In support of this later hypothesis, there is evidence that orthologues of the autoantigen Ro60 in the gut microbiota cross-react with human B cells and T cells, thus stimulating autoimmune diseases in a mechanism involving Ruminococcus gnavus.11 Thus, changes in the gut microbiota, the immune system and SLE appear to be related.
Although many studies have examined the association between SLE and gut microbiota, some of the results are still controversial, such as the reported decrease in alpha diversity and changes in the abundance of some gut microbes in patients with SLE. For example, Bellocchi et al12 and WF et al13 reported different results regarding alterations in the Shannon-Wiener diversity index between patients with SLE and HCs. A change in the abundance of Enterobacteriaceae in patients with SLE is also uncertain.12 14 Because the composition of the gut microbiota is dynamic and is affected by genes, diet, geographical region, use of immunosuppressants and other factors,15–20 there could be many explanations for the
differences in these experimental results. In addition, differences in the design of experiments and methods used to study the gut microbiota can also impact the results.21
Thus, we examined the changes in the gut microbiota of patients with SLE and the factors that may affect these changes by performing a meta-analysis of relevant research publications from around the world in an effort to provide an evidence-based approach for follow-up research.
Methods
This meta-analysis was based on the procedures of the Meta-analysis of Observational Studies in Epidemiology group, which provides meta-guidance for observational studies.22 The present study was registered in PROSPERO (International Prospective Record of Systematic Reviews) on 17 May 2021 (CRD42021249607).
Literature search
Two researchers (SX and YQ) conducted the literature search using the following English and Chinese databases: PubMed, Embase, Cochrane, Web of Science, Wanfang Database and Chinese National Knowledge Infrastructure (http://www.cnki.net). Combinations of medical subject headings and synonyms were used to search for studies (online supplemental file 1). The literature search was finalised on 1 March 2021.
Supplemental material
Study selection criteria
All included studies performed research at the population level; examined patients in a case group who were adults diagnosed with SLE based on the American College of Rheumatology (ACR) criteria; compared changes in the gut microbial diversity, or the relative abundance of microbes, in patients with SLE and HCs; examined faecal samples; and had data available for analysis. Studies were excluded if they were animal experiments, provided irrelevant research content, were review articles or failed to provide complete experimental data.
Data extraction
The following characteristics were recorded from the selected studies: (1) first author, year of publication, and country and region where the study was performed; (2) characteristics of the subjects (sex, average age, population, current use of medications and doses); (3) experimental methods (diagnostic criteria, sample size, techniques for assessment of the gut microbiota); (4) alpha diversity of the gut microbiota in patients and HCs; (5) abundance of different gut microbial species, which was expressed as the mean proportion of each microbiota; and (6) results reported as mean and respective SD.
In addition, since beta diversity of the gut microbiota in patients and HCs was depicted with a principal coordinates analysis plot, we were unable to extract data for our study. Therefore, this study did not perform a meta-analysis of beta diversity.
Data extraction and transformation
The original data from each publication were extracted for the meta-analysis and the results are expressed as tables and graphs. Because some publications provided relevant data indirectly, GetData Graph Digitizer V.2.25 (http://getdata-graph-digitizer.com) was used to extract data from plots23–25 using the methods described by Wan et al.26
Quality assessment
Two researchers (SX and YQ) independently extracted relevant data from the included studies. Disagreements were resolved through discussion and consultation with a third researcher (SQ). The nine-star Newcastle-Ottawa Quality Assessment Scale (NOS) for case–control studies was used to assess the quality of all studies.27 This scale considers adequate definition of cases, representativeness of cases, selection of controls, definition of controls, ascertainment of exposure, same method of ascertainment for cases and controls, and non-response rate. The highest possible score was 9. Studies with scores of 6–9 points (high-quality publications) were included in the meta-analysis.
Statistical analysis
The collected or converted data (including the Shannon-Wiener diversity index, Chao1 richness estimator and abundance of specific gut microbes) were collated. When four or more studies examined the same outcome, data were analysed using Stata V.16.0 software and the results were displayed as forest plots. When the data included were continuous variables and the measurement methods were the same, weighted mean differences (WMDs) were used as the effect scale. When there was a large difference in mean or SD among the included studies, standardised mean differences (SMDs) were used as the effect scale.28
Statistical heterogeneity was evaluated using the χ2-based Q statistic test. I2 was used as an index to evaluate heterogeneity and was defined as moderate (25%), large (50%) or extreme (75%).29 Direct meta-analyses were performed using a sample-size weighted random effects model.30 An I2 greater than 25% was considered to indicate statistically significant heterogeneity. A p value below 0.05 was considered significant.31 However, since our study has been tested seven times, a Bonferroni correction was considered. A p value <0.0071 meets the Bonferroni threshold of statistical significance (0.0071=0.05/7). Results with p value >0.0071 were not considered statistically significant.32
Subgroup analyses were performed to determine the effect of population and use of medications. Sensitivity analysis was performed to evaluate the effect of individual studies on the overall results by deleting one study at a time and combining the effect values of the remaining studies.
Due to the limited number of studies ultimately included, standard bias assessment results may not be sufficiently reliable. Nonetheless, publication bias was evaluated by Egger’s test,33 Begg’s test34 and a funnel plot. The Egger’s and Begg’s tests indicated no publication bias when the p value was greater than 0.05, and otherwise indicated publication bias. When the results of Egger’s and Begg’s tests were inconsistent, the results of the Egger’s test were used as the reference value.
Results
Study selection and characteristics
We initially retrieved 720 potentially eligible studies (figure 1). We then deleted duplicate publications using EndNote, and also deleted publications that were unrelated to the research topic, publications that examined animals and review articles. After reading the full text of the remaining publications, we deleted publications that provided incomplete experimental results, unclear experimental procedures or examined faecal samples that were not from humans. The remaining 31 studies reported Shannon-Wiener diversity indexes, Chao1 richness estimators and abundance of some gut microbes. Twenty-four studies remained after deleting eight studies where the data could not be collected, or where the gut microbiota were described as log10 bacteria per gram of faeces or as a linear discriminant analysis score. After quality evaluation, we ultimately included 11 studies in the meta-analysis, 9 English-language studies and 2 Chinese-language studies (table 1).
These 11 studies examined 373 patients with SLE and 1288 HCs. All 11 studies used human faeces as experimental samples, used diagnostic criteria for SLE from the ACR and were observational case–control studies. According to the NOS, each of the included studies had high quality (NOS score: 6–8; table 2).
Changes in alpha diversity of gut microbes in patients with SLE
We compared the Shannon-Wiener diversity index and the Chao1 richness estimator for the SLE and HC groups to determine the relationship of SLE with alpha diversity and overall richness of the gut microbiota. Because there was a difference of more than 10 times in the average values of other indicators except for the Shannon-Wiener diversity index, we used SMD as the effect scale. Analysis of the Shannon-Wiener diversity index showed that the studies had no significant heterogeneity (I2=0%, p>0.01) and that the SLE group had a lower diversity of gut microbiota than the HC group (WMD=−0.22, 95% CI −0.32 to –0.13, p<0.001, n=10; figure 2A). There was significant heterogeneity among the studies in the Chao1 richness estimator (I2=70.2%, p<0.01). After sensitivity analysis, we removed the study of Zhu,13 and this eliminated the heterogeneity in Chao1 richness estimator (figure 3A). Analysis of the remaining studies indicated lower richness of the gut microbiota in the SLE group than in the HC group (SMD=−0.84, 95% CI −1.07 to –0.61, p<0.001, n=5; figure 3A).
We used the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI)35 to group patients into an active SLE group (SLEDAI ≥8) and an SLE remission group (SLEDAI <8). Subgroup analysis of these two groups indicated no significant difference in the Shannon-Wiener diversity index in the SLE remission and SLE active groups (p>0.05; figure 4A).
Because the prevalence of SLE is much higher in women than in men, we conducted a subgroup analysis to examine the effect of gender. The results showed that the decrease in the Shannon-Wiener diversity index of male and female patients with SLE together was greater than HCs (WMD=−0.26, 95% −0.39 to –0.14, p<0.001, n=7). However, there was no significant difference between female patients with SLE and HCs (WMD=−0.16, 95% −0.32 to 0.00, p=0.052, n=3; figure 4B).
Changes in specific gut microbes in SLE
We then analysed the different taxa in the gut microbiota of patients with SLE and HCs. The results indicated the SLE group had a decreased abundance of Ruminococcaceae (, (SMD = −0.49, 95% CI = −0.84 to –0.15, p=0.005, n=5; figure 2C) and an increased abundance of Enterobacteriaceae (SMD=0.45, 95% CI 0.01 to 0.89, p=0.045, n=5; figure 2D). However, the results for Enterobacteriaceae were not statistically significant according to the Bonferroni statistical significance threshold (p>0.0071). In addition, the SLE and HC groups had no significant differences in Lachnospiraceae, Enterococcaceae or Bacteroides (all p>0.05).
Due to the significant heterogeneity among the studies, we used sensitivity analysis to evaluate the source of this heterogeneity (online supplemental file 2). After removing the van der Meulen et al study8 and the Bellocchi et al study,12 the heterogeneity in Chao1 richness estimator, Enterobacteriaceae (figure 3B), Lachnospiraceae (figure 3C), Enterococcaceae (figure 3D) and Bacteroides (figure 3E) decreased or disappeared, but the results for Lachnospiraceae (p=0.327) and Bacteroides (p=0.756) were unaffected. However, after sensitivity analysis, the p value for Enterococcaceae indicated a significant difference (SMD=0.53, 95% CI 0.05 to 1.01, I2=43.9%, p=0.03, n=3; figure 3D). In other words, the abundance of Enterococcaceae in the SLE group was greater than in the HC group, but the results did not strictly meet the multiple comparisons criterion (p>0.0071). Removing some of the included studies did not reduce the statistical heterogeneity in Ruminococcaceae. In addition, after sensitivity analysis, the heterogeneity in Enterococcaceae was still higher than 25%.
Supplemental material
We also performed a subgroup analysis with stratification by population and use of medications. The abundance of Ruminococcaceae in patients with SLE was lower than in HCs (SMD=−0.90, 95% CI −1.34 to –0.45, p<0.001; table 3), but this difference was not significant for the non-Chinese subgroup (SMD=−0.22, 95% CI=−0.58 to 0.13, p=0.216; table 3). In the Chinese subgroup, the abundance of Enterococcaceae in patients with SLE was higher than in HCs (SMD=0.77, 95% CI 0.34 to 1.21, p=0.001; table 4), but there were no differences between the non-Chinese subgroups (SMD=−0.08, 95% CI −0.49 to 0.32, p=0.682; table 4). The use of medications had inconsistent effects in these different taxonomic groups.
Analysis of publication bias
We assessed the risk of publication bias using Egger’s and Begg’s tests and based on the symmetry of a funnel chart (online supplemental file 2, figure S1). The results indicated no evidence of publication bias, indicating the conclusions of the meta-analysis were relatively robust.
Discussion
Our meta-analysis compared the gut microbiota of patients with SLE and HCs and led to three major conclusions. First, patients with SLE had a lower diversity of gut microbiota, and the Shannon-Wiener diversity index was slightly lower in patients with active SLE than in those in remission. Second, analysis of the different taxonomic groups of gut microbes indicated that patients with SLE had a decreased abundance of Ruminococcaceae, increased abundance of Enterobacteriaceae and Enterococcaceae, and no significant changes in Lachnospiraceae and Bacteroides. Third, changes in Ruminococcaceae and Enterococcaceae in patients with SLE were greater in Chinese patients with SLE.
The Chao1 richness estimator is simply an indicator of the overall number of species richness,36 whereas the Shannon-Wiener diversity index is an indicator of the uniformity of the number of different species.37 Our results indicated that both parameters were decreased in the gut microbiota of patients with SLE. As a result, we posit that the gut microbiota of these patients appear to have reduced stability, altered structure and function, and reduced ability to resist changes. Our comparison of patients with SLE in active and remission with HCs indicated no significant effect on the Shannon-Wiener diversity index. This result seems to contradict the findings of Azzouz et al.38 There was significant heterogeneity among the studies in the SLE active groups and a larger sample size may change the result. In addition, we found that the Shannon-Wiener diversity index was lower for all subjects (men and women) than women alone. This preliminary finding suggests a need for further research on the effect of gender on the gut microbiota of patients with SLE.
Our findings of a decreased abundance of Ruminococcaceae in patients with SLE differed from the results of Wen et al.39 Microbes in this family can produce short-chain fatty acids (SCFAs),25 which are considered potential ‘orchestrators’ of the cross-talk between the gut microbiota and the host metabolism.40 A reduction of these microbes may therefore lead to reduced SCFA production and disruptions of human metabolism. Some reports found that SCFAs can inhibit B cell activation-induced cytidine deaminase (AID) and Blimp1 expression, plasma cell differentiation and class-switching by autoantibodies’ class switching; prevent IgG1/IgG2 deposition in the kidney; and prevent lupus skin lesions.41 Therefore, a decreased abundance of species in Ruminococcaceae may be responsible for some of the complications experienced by patients with SLE. In addition, SCFAs are the main energy source of colon cells,42 and these protect the integrity of the small intestinal epithelial cell membrane.43 44 There is also evidence that a decreased in the abundance of Ruminococcaceae may also lead to ‘leaky gut’ (increased intestinal permeability).
Enterobacteriaceae is in the Proteobacteria phylum and is a large family in which many species are pathogens or produce inflammatory reactions. Enterobacteriaceae are among the most common pathogens responsible for abdominal infections, and the production of extended-spectrum beta-lactamases is the main mechanism of their pathogenesis.45 Previous research showed that a high level of S100A8-A9 (a calcium-binding extracellular complex) in the intestines of infants can reduce the abundance of intestinal Enterobacteriaceae through the expansion of Tregs, and thereby promote the healthy development of intestinal microflora.46 The abundance of Enterobacteriaceae is also associated with T cells, and this may be the mechanism by which changes in the abundance of Enterobacteriaceae affect the occurrence and development of SLE. Enterobacteriaceae are also among the main pathogens responsible for pulmonary infections.47 Thus, the increased abundance of Enterobacteriaceae in the gut of patients with SLE may be one of the factors that contribute to multiple infections of the lungs and abdominal cavity in these patients.
Enterococcaceae are generally considered a beneficial microflora because they function in reducing gastrointestinal damage.48 However, species in this family may also contribute to the development of cancer.49 Further studies are needed to determine whether the increase of Enterococcaceae in patients with SLE is a compensatory response to restore gut homeostasis25 50 or whether it leads to tumourigenesis.
However, there is a risk of false-positives in the results for Enterobacteriaceae and Enterococcaceae after correction for multiple comparisons. The risk may be due to insufficient sample size51 as these two groups of gut microbes had the smallest sample sizes of all the positive samples. Therefore, further studies with a larger sample size are necessary to confirm our results.
It is important to consider technical methods used to assess the gut microbiota in the 11 different studies in our meta-analysis. The study by Chen et al52 was the only study to use shotgun sequencing, and all the others relied on 16S rRNA sequencing. This later method provides information on phylogenetic abundance up to the genus level, but has low taxonomic resolution at the species level.53 Notably, the metagenome-wide study of Chen et al52 found an increased level of R. gnavus in patients with SLE. There is evidence that intestinal expansion of this species leads to cross-reaction with lupus anti-double stranded DNA (dsDNA) antibodies and correlated with lupus nephritis.38 Bagavant et al54 also found an increase in Enterococcus gallinarum in patients with SLE and that this was significantly associated with the presence of anti-dsDNA and anti-Sm autoantibodies. However, we cannot infer changes at the level of the species or genus based on changes at the level of the family, as determined by 16S RNA data.
We also examined the effect of medications on the gut microbiota of patients with SLE using sensitivity analysis. Previous research showed that glucocorticoid therapy similarly altered the diversity of operational taxonomic units in patients with SLE and HCs in that it restored the ratio of Firmicutes to Bacteroidetes.55 Our study indicated that medication use may affect the Chao1 index. Guo et al55 reported the gut microbiota was similar in HCs and patients with SLE who received prednisone doses up to 20 mg. None of the studies we included examined patients who received glucocorticoid doses above 10 mg/day, and this may be the reason for our discrepant results. Studies of mice with experimentally induced SLE also support the argument that a high dose of prednisone (10 mg/kg) can change the Shannon-Weiner index and Chao1 estimator of the gut microbiota.56 Another study reported that administration of different doses of corticosteroids to mice with SLE led to changes in different bacterial taxa and alterations in metabolic functions.56 Thus, changes in the gut microbiota of patients with SLE may be used as a reference for clinical adjustment of the glucocorticoid dose because this can be difficult to adjust in the clinic.
Short-term/high-dose or long-term use of hydroxychloroquine (HCQ) can also alter the gut microbiota, resulting in a decreased level in the relative abundance of Firmicutes.57 58 For example, Bellocchi et al12 examined patients with SLE, 70.37% of whom were taking HCQ. HCQ can inhibit the stimulation of Toll-like receptors59 and reduce the level of interleukin 1 and 6, thus achieving anti-inflammatory effects. This previous study found that HCQ combined with hormone therapy may cause a decrease in the abundance of Enterobacteriaceae compared with glucocorticoids or HCQ alone, a possible mechanism by which HCQ can reduce the risk of infection rate in patients with SLE. In addition, HCQ may also cause a decrease in the abundance of Enterococcaceae, which may be one of the mechanisms for its gastrointestinal adverse effects.60
In addition to disease, diet and environment, race and ethnicity can also impact the gut microbiota.61 62 For example, the van der Meulen et al study8 may have been a source of heterogeneity in our meta-analysis due to the different backgrounds of the included population; 28 were white/European, but none of the HCs was white/European. Thus, there was a significant decrease in Ruminococcaceae in the faeces of patients with SLE from Spain, but this phenomenon was not evident in Chinese patients.63 Some other research suggested an influence of genetic background on the gut microbiota. For example, Goodrich et al64 analysed the gut microbiota in 1126 pairs of British twins and found that Christensenellaceae was the most heritable taxon. It is possible that Ruminococcaceae and Enterococcaceae families may also be heritable.
Chen et al52 and and Tomofuji et al53 used a metagenome-wide approach to identify the high abundance of Streptococcaceae and Streptococcus in patients with SLE, and also analysed their results at the level of genes, biological pathways and functional level. However, most of our results were based on studies that used 16S rRNA sequencing, which has limitations. Thus, we could not analyse microbes at the genus level, such as Streptococcus and Veillonella. We anticipate that future research on this topic will increasingly use a metagenome-wide approach. The lack of using compositional data analysis is a limitation of these studies, which may produce errant results and spurious correlations.65 Another limitation is that very little is known about the effects of glucocorticoid dose on the abundance and metabolomics of gut microbiota, so our meta-analysis could not examine this important topic. In addition, we were unable to analyse the changes in the gut microbiota between different genders due to the limitations of the study population. Also, different studies used different reference panels (standard 16S rRNA databases used for alignment), which may lead to different inferences, confounding and heterogeneity.
In conclusion, our meta-analysis summarised the differences in the gut microbiota between patients with SLE and HCs, including the changes in diversity and in the relative abundance of different taxa. Our findings suggest that targeted alteration in the abundance of intestinal flora has potential for the treatment of SLE. In addition, we also found that medication use can affect the gut microbiota.
Data availability statement
Data are available upon reasonable request.
Ethics statements
Patient consent for publication
Ethics approval
Not applicable.
Acknowledgments
This article was already published as a non-peer reviewed article in Research Square (DOI 10.21203/rs.3.rs-608010/v1).
References
Supplementary materials
Supplementary Data
This web only file has been produced by the BMJ Publishing Group from an electronic file supplied by the author(s) and has not been edited for content.
Footnotes
SX, YQ and SQ contributed equally.
Contributors Conceptualisation: SX and YQ. Methodology: SQ. Software: SX. Validation: YQ and SQ. Formal analysis: SX. Investigation: RW and YJ. Resources: RW and YJ. Data curation: RW. Writing - original draft preparation: SX. Writing - review and editing: SX and YQ. Visualisation: SQ. Supervision: XD. Project administration: XD. Funding acquisition: XD. All authors have discussed the results and contributed to the writing of the manuscript and approved the final manuscript. XD is responsible for the overall content as guarantor.
Funding This research was funded by the National Natural Science Foundation of China (no: 81774179 and 81973778).
Competing interests None declared.
Provenance and peer review Not commissioned; externally peer reviewed.
Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise.