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Thursday 06 October 2022 from 13:00 to 14:10
Po.3 E- poster session 3: cardiovascular manifestations, cytokines and complement system in SLE, difficult cases, organ damage and survival, innate immunity
PO.3.66 TLR9 protein level is associated to proinflammatory cytokine level of IL10 and inf1a in SLE patients
  1. E Grau García1,
  2. L Gomez-Lechón Quirós2,
  3. C Riesco Barcena1,
  4. AV Huaylla Quispe1,
  5. S Leal Rodríguez1,
  6. C Pávez Perales1,
  7. L Mas Sanchez1,
  8. P Muñoz Martínez1,
  9. M De La Rubia Navarro1,
  10. I Cánovas Olmos1,
  11. J Ivorra Cortés1,
  12. JJ Fragio Gil1,
  13. L González Puig1,
  14. I Martínez Cordellat1,
  15. C Nájera Herranz1,
  16. R Negueroles Albuixech1,
  17. JE Oller Rodríguez1,
  18. FM Ortiz-Sanjuán1,
  19. E Vicens Bernabéu1 and
  20. JA Roman Ivorra1
  1. 1Rheumatology Department. HUP La Fe. ~ Valencia ~ Spain
  2. 2Rheumatology Department. Hospital Francesc de Borja ~ Gandia ~ Spain

Abstract

Purpose The aim is to investigate the association among TLR7 and TLR9 serum levels with previous viral infections, disease activity and proinflammatory cytokine levels in SLE patients.

Methods Cross-sectional observational study in SLE patients (SLICC/ACR 2012 criteria) and healthy controls (HC). Previous infection data with RNA (HCV) and DNA virus (CMV, Epstein-Barr, Herpes simplex, Parvovirus B19 or HBV), disease activity and clinical data were collected. Biological samples of SLE patients and HC from the medical visit were supplied to the TLR7, TLR9, IL10 and INF1A determination by enzyme-linked immunoassay.

Results 94 SLE patients (91.5% female) with a mean age of 51 (13) years old and 35 HC (80% female) and 42 (12) years old were recruited. Mean age at diagnosis was 33 (14) years old and mean disease evolution was 19 (10) years. Mean SLEDAI index was 5.35 (4.58).

The 48.94% of patients reported almost one DNA virus infections, the 2.13% reported HCV infection, the 4.25% with HCV and DNA virus, and the 31.92% not reported any infection. HC had no history of acute (3 months) or lasting chronic infections with viruses of bacteria.

TLR7 and TLR9 did not correlate between them. TLR9 levels were significantly higher in SLE patients than HC (P<0,001). Even though TLR7 levels did not show any difference between both groups, an association with the age of individuals was observed (P<0,001).

No association among TLR7 or TLR9 levels with CRP, ESR, anti-dsDNA, ENAs or antiphospholipid antibodies was observed, and nor with disease activity, age at diagnosis and disease evolution time. In contrast, however, we reported low TLR7 levels in SLE patients and antiphospholipid syndrome in comparison to those without antiphospholipid syndrome (P=0,001).

High TLR9 levels were significantly associated to increased levels of IL10 and INF1A in SLE patients (P<0,001). TLR7 levels were not associated with INF1A levels but it is noticeable that there is a tendency to increase TLR7 levels in cases with increase of IL10 levels.

Conclusions TLR9 is increased in SLE patients in comparison to HC. TLR7 increases with age. No evidence of association between previous infections and TLR levels was found. Nor do we observe any difference in TLR level according to autoantibodies presence or disease activity, probably due to the long-term SLE evolution and a good control of the disease. There was, however, an association between high TLR9 levels and increase of IL10 and INF1A.

http://creativecommons.org/licenses/by-nc/4.0/

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

http://dx.doi.org/10.1136/lupus-2022-elm2022.95

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