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Lupus Nephritis
1106 Stability of novel urinary biomarkers used for lupus
  1. Ellen Cody1,
  2. James Rose1,
  3. Rebecca Hopkins1,
  4. Megan Quinlan-Waters2,
  5. Catherine Robben2,
  6. Tingting Qiu3,
  7. Bin Huang3,4,
  8. Hermine Brunner2,4 and
  9. Prasad Devarajan1,4
  1. 1Cincinnati Children’s Hospital, Division of Nephrology and Hypertension, USA
  2. 2Cincinnati Children’s Hospital, Division of Rheumatology, USA
  3. 3Cincinnati Children’s Hospital, Division of Epidemiology and Biostatistics, USA
  4. 4University of Cincinnati, Department of Pediatrics

Abstract

Background We have developed and validated the Renal Activity Index for Lupus (RAIL), a composite score of six urinary biomarkers including neutrophil gelatinase – associated lipocalin (NGAL), monocyte chemoattractant protein-1 (MCP-1/CCL2), kidney injury molecule-1 (KIM-1), ceruloplasmin, adiponectin, and hemopexin) to monitor disease activity. It is critical to establish optimal sample handling conditions and storage prior to widespread clinical deployment and meaningful use in clinical trials. We have previously demonstrated the excellent short-term storage stability of NGAL and KIM-1; here we expand testing to include the other 4 RAIL biomarkers.

Methods Urine was collected from 10 patients enrolled in the SLE Clinical and Research Database (IRB 2008-0635). The urine was then aliquoted and tested under shipping conditions, including freeze/thaw, ambient and longer-term storage (figure 1). MCP-1, Ceruloplasmin, Adiponectin and Hemopexin were assayed by single-plex ELISA assay via commercially available kits. We performed Pearson Correlation Coefficient, Deming regression and Bland-Altman analysis.

Results There was no statistical difference in biomarker concentrations in any of the four biomarkers in any of the experimental conditions. Urinary MCP-1, Adiponectin, Hemopexin and Ceruloplasmin are stable following storage at -80°C for up to 3 months, and at 4° or 25°C up to 48 hours followed by -80° C. In addition, shipping on dry ice or with refrigeration leads to no significant loss of signal. The addition of 1 or 2 additional freeze thaw cycles also did not change mean biomarker levels.

Conclusions RAIL biomarkers are stable following short-term storage at clinically relevant conditions, including shipping on ice.

Abstract 1106 Figure 1
Abstract 1106 Figure 1
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Abstract 1106 Table 1
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This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ .

http://dx.doi.org/10.1136/lupus-2022-lupus21century.71

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