@article {Renauere000101, author = {Paul Renauer and Patrick Coit and Matlock A Jeffries and Joan T Merrill and W Joseph McCune and Kathleen Maksimowicz-McKinnon and Amr H Sawalha}, title = {DNA methylation patterns in na{\"\i}ve CD4+ T cells identify epigenetic susceptibility loci for malar rash and discoid rash in systemic lupus erythematosus}, volume = {2}, number = {1}, elocation-id = {e000101}, year = {2015}, doi = {10.1136/lupus-2015-000101}, publisher = {Archives of Disease in childhood}, abstract = {Objective Systemic lupus erythematosus (SLE) is a complex autoimmune disease characterised by heterogeneous clinical manifestations, autoantibody production and epigenetic dysregulation in T cells. We sought to investigate the epigenetic contribution to the development of cutaneous manifestations in SLE.Methods We performed genome-wide DNA methylation analyses in patients with SLE stratified by a history of malar rash, discoid rash or neither cutaneous manifestation, and age, sex and ethnicity matched healthy controls. We characterised differentially methylated regions (DMRs) in na{\"\i}ve CD4+ T cells unique to each disease subset, and assessed functional relationships between DMRs using bioinformatic approaches.Results We identified 36 and 37 unique DMRs that contribute to the epigenetic susceptibility to malar rash and discoid rash, respectively. These DMRs were primarily localised to genes mediating cell proliferation and apoptosis. Hypomethylation of MIR886 and TRIM69, and hypermethylation of RNF39 were specific to patients with SLE with a history of malar rash. Hypomethylation of the cytoskeleton-related gene RHOJ was specific to patients with SLE with a history of discoid rash. In addition, discoid rash-specific hypomethylated DMRs were found in genes involved in antigen-processing and presentation such as TAP1 and PSMB8. Network analyses showed that DMRs in patients with SLE with but not without a history of cutaneous manifestations are associated with TAP-dependent processing and major histocompatibility-class I antigen cross-presentation (p=3.66{\texttimes}10-18 in malar rash, and 3.67{\texttimes}10-13 in discoid rash).Conclusions We characterised DNA methylation changes in na{\"\i}ve CD4+ T cells specific to malar rash and discoid rash in patients with SLE. These data suggest unique epigenetic susceptibility loci that predispose to or are associated with the development of cutaneous manifestations in SLE.}, URL = {https://lupus.bmj.com/content/2/1/e000101}, eprint = {https://lupus.bmj.com/content/2/1/e000101.full.pdf}, journal = {Lupus Science \& Medicine} }