PT  - JOURNAL ARTICLE
AU  - Evan Der
AU  - Hemant Suryawanshi
AU  - Jill Buyon
AU  - Thomas Tuschl
AU  - Chaim Putterman
TI  - Single-cell RNA sequencing for the study of lupus nephritis
AID  - 10.1136/lupus-2019-000329
DP  - 2019 Jun 01
TA  - Lupus Science & Medicine
PG  - e000329
VI  - 6
IP  - 1
4099  - http://lupus.bmj.com/content/6/1/e000329.short
4100  - http://lupus.bmj.com/content/6/1/e000329.full
SO  - Lupus Sci Med2019 Jun 01; 6
AB  - Single-cell RNA sequencing (scRNA-seq) has recently undergone rapid advances in the development of this technology, leading to high throughput and accelerating discovery in many biological systems and diseases. The single-cell resolution of the technique allows for the investigation of heterogeneity in cell populations, and the pinpointing of pathological populations contributing to disease. Here we review the development of scRNA-seq technology and the analysis that has evolved with the ever-increasing throughput. Finally, we highlight recent applications of scRNA-seq to understand the molecular pathogenesis of lupus and lupus nephritis.