PT - JOURNAL ARTICLE AU - Der, Evan AU - Suryawanshi, Hemant AU - Buyon, Jill AU - Tuschl, Thomas AU - Putterman, Chaim TI - Single-cell RNA sequencing for the study of lupus nephritis AID - 10.1136/lupus-2019-000329 DP - 2019 Jun 01 TA - Lupus Science & Medicine PG - e000329 VI - 6 IP - 1 4099 - http://lupus.bmj.com/content/6/1/e000329.short 4100 - http://lupus.bmj.com/content/6/1/e000329.full SO - Lupus Sci Med2019 Jun 01; 6 AB - Single-cell RNA sequencing (scRNA-seq) has recently undergone rapid advances in the development of this technology, leading to high throughput and accelerating discovery in many biological systems and diseases. The single-cell resolution of the technique allows for the investigation of heterogeneity in cell populations, and the pinpointing of pathological populations contributing to disease. Here we review the development of scRNA-seq technology and the analysis that has evolved with the ever-increasing throughput. Finally, we highlight recent applications of scRNA-seq to understand the molecular pathogenesis of lupus and lupus nephritis.