TY - JOUR T1 - Single-cell RNA sequencing for the study of lupus nephritis JF - Lupus Science & Medicine JO - Lupus Sci Med DO - 10.1136/lupus-2019-000329 VL - 6 IS - 1 SP - e000329 AU - Evan Der AU - Hemant Suryawanshi AU - Jill Buyon AU - Thomas Tuschl AU - Chaim Putterman Y1 - 2019/06/01 UR - http://lupus.bmj.com/content/6/1/e000329.abstract N2 - Single-cell RNA sequencing (scRNA-seq) has recently undergone rapid advances in the development of this technology, leading to high throughput and accelerating discovery in many biological systems and diseases. The single-cell resolution of the technique allows for the investigation of heterogeneity in cell populations, and the pinpointing of pathological populations contributing to disease. Here we review the development of scRNA-seq technology and the analysis that has evolved with the ever-increasing throughput. Finally, we highlight recent applications of scRNA-seq to understand the molecular pathogenesis of lupus and lupus nephritis. ER -