Overview of anti-dsDNA tests used in the participating laboratories for analysis of serum aliquots from all patients
| Name | Methodology | Antigen | Reference interval | Manufacturer | Centre |
|---|---|---|---|---|---|
| CLIFT 1 | IIF | Crithidia luciliae kinetoplast | <titre 10 | ImmunoConcept | CPH |
| CLIFT 2 | IIF | Crithidia luciliae kinetoplast | <titre 10 | ImmunoConcept | Tromsø |
| CLIFT 3 | IIF | Crithidia luciliae kinetoplast | <titre 10 | Euroimmune | Lund |
| EliA | Solid phase ELISA | Recombinant plasmid dsDNA | <10 IU/mL | Phadia | Tromsø |
| SPADE | Solution phase ELISA | Biotinylated plasmid dsDNA and biotinylated, S1 nucleased human dsDNA | <1 AU/mL | Inhouse | Tromsø |
| Varelisa 1 | Solid phase ELISA | Recombinant plasmid dsDNA | <55 IU/mL | Phadia | Tromsø |
| Varelisa 2 | Solid phase ELISA | Recombinant plasmid dsDNA | <35 IU/mL | Phadia | CPH |
AU, arbitrary units; CLIFT, Crithidia Lucillia Immunofluorescence Test; CPH, Copenhagen; IIF: indirect immunofluorescence technique; IU, international units; SPADE, solution phase anti-dsDNA ELISA.