Quantitative and qualitative comparison of direct and indirect magnetic bead-based CD4+ T-cell isolation kits with subsequent bead-based DNA isolation
| Parameter | CD4 MicroBeads kit (direct) | CD4+T cell isolation kit (indirect) | p Value | Hedges' g (95% CI) |
|---|---|---|---|---|
| A260/A280 | 1.82±0.05 | 1.78±0.03 | 0.0488 | 0.88 (−0.03 to 1.80) |
| c(DNA) (µg/mL) | 59.55±27.42 | 53.00±19.95 | 0.0924 | 0.26 (−0.62 to 1.14) |
| c(DNA) (ng/(mlxcell)) | 50.97±14.15 | 49.53±13.62 | 0.4922 | 0.10 (−0.78 to 0,98) |
| Purity CD3+ (%) | 93.11±1.47 | 94.99±1.54 | 0.1250 | −1.1 (−2.46 to 0.21) |
p-value according to Wilcoxon matched-pairs signed-rank test (significant results with p<0.05 printed in bold), effect size estimated by Hedges' G with 95% CI.