Effect of an inhibitor of DNA methylation on T cells. II. 5-azacytidine induces self-reactivity in antigen-specific T4+ cells

doi:10.1016/0198-8859(86)90304-6

Human Immunology

Volume 17, Issue 4, December 1986, Pages 456-470

https://doi.org/10.1016/0198-8859(86)90304-6 Get rights and content

Abstract

During T-cell maturation, thymocytes interact with thymic stromal major histocompatibility complex (MHC) determinants and thymic hormones, and proliferate, apparently in response to MHC gene products, in the absence of antigen. The maturing thymocytes also express a series of cell surface molecules, at one stage coexpressing T4, T6, and T8. Mature T cells express either T4 or T8, lack T6, bear the T3-Ti receptor complex on the cell surface, and require antigen in addition to MHC determinants to proliferate. To study whether DNA methylation may be involved in regulating phenotypic and functional changes observed during thymocyte maturation, cloned, T4⁺ Interleukin-2 dependent, antigen-specific T cells were treated with an inhibitor of DNA methylation, 5-azacytidine (5-azaC). The 5-azaC treated cells lost the requirement for antigen and could be activated by autologous macrophages alone. Anti-class II and anti-T3, but not anti-class I monoclonal antibodies, inhibited activation of 5-azaC treated T4⁺ cells by macrophages, implying that the T3-Ti receptor complex may be recognizing class II MHC molecules without antigen. No changes in T3 and T4 expression were noted, and neither T8 nor T6 was induced.

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Effect of an inhibitor of DNA methylation on T cells. II. 5-azacytidine induces self-reactivity in antigen-specific T4+ cells

Abstract

Cell

Cell

Cell

Cell

Cell

Biochem Biophys Acta

Biochem Biophys Res Comm

Adv Immunol

Intrathymic lymphopoiesis: stromal cell-associated proliferation of T cells is independent of lymphocyte genotype

J Immunol

Thymocyte and macrophage interactions: separation of murine thymocyte subsets and enrichment of syngeneic cell-responding thymocytes by absorption to macrophage monolayers

J Immunol

Thymic T cells are driven to expand upon interaction with self-class II major histocompatibility complex gene products on accessory cells

Thymosins: structure, function and therapeutic applications

Thymus

Thymic function in man

Thymus

The differentiation of T lymphocytes. V. Evidence for intrathymic death of most thymocytes

Thymus

DNA methylation and regulation of the human β-globin like genes in mouse erythroleukemia cells containing human chromosome 11

Methylation patterns of immunoglobulin genes in lymphoid cells: correlation of expression and differentiation with undermethylation

Preferential binding of estrogen-receptor complex to a region containing the estrogen-dependent hypomethylation site preceding the chicken vitallogenin II gene

Molecular mechanism of human hemoglobin switching: selective undermethylation and expression of globin genes in embryonic, fetal, and adult erythroblasts

DNA methylation of three 5′ C-C-G-G 3′ sites in the promoter and 5′ region inactivate the E2a gene of adenovirus type 2

Pholman: Activation of a chicken embryonic globin gene in adult erythroid cells by 5-azacytidine and sodium butyrate

Clonal inheritance of the pattern of DNA methylation in mouse cells by 5-azacytidine and sodium butyrate

Covalent bond formation between a DNA-cytosine methyltransferase and DNA containing 5-azacytosine

Induction of the metastatic phenotype in a mouse tumor model by 5-azacytidine, and characterization of an antigen associated with metastatic activity

The use of 5-azacytidine to establish constitutive interleukin-2 producing clones of the EL4 thymoma

J Immunol

Deriviation of macrophage-like lines from the pre-B lymphoma ABLS 8.1 using 5-azacytidine

Nature

Differentiation of myoblast cell lines and biological methylation: 3-deazaadenosine stimulates formation of multinucleated myofibers

Isolation of lymphocytes, granulocytes and macrophages

Scand J Immunol

The maintenance of B-cell and T-cell function in frozen and stored human lymphocytes

J Clin Immunol

Spontaneous release of a factor with properties of T cell growth factor from a continuous line of primate tumor T cells

J Immunol

New standard for cellular immunology

Am Clin Prod Rev

Effect of an inhibitor of DNA methylation on T cells. II. 5-azacytidine induces self-reactivity in antigen-specific T4⁺ cells