A noninvasive method for quantifying and distinguishing inflammatory skin reactions☆,☆☆
Section snippets
Induction of erythematous reactions on the skin
The ICD was induced by applying 0.5% sodium lauryl sulfate (SLS, Fisher Scientific, San Diego, Calif) in distilled water for 72 hours. At this point, the erythema was graded according to a standard scoring scale and tape stripping was performed (see below).12 Dibutyl squarate (2%) in acetone was applied for 48 hours under occlusion to the upper arm to induce the ACD reactions. After about 14 days the dibutyl squarate (2%) was reapplied to elicit the immune-mediated reaction. The scoring of the
Cytokines identified by RPA
We initially used 4 RNA probes (IL-4, IL-8, L32, GAPDH) for hybridization to RNA samples obtained from 1 individual. Fig 1 shows the cytokines identified by RPA analysis of the cells obtained by tape stripping (12 times) skin sites on the upper arms of this individual (the same individual as subject 2 in Fig 2).
DISCUSSION
These results demonstrate that skin RNA can be obtained from tape strips without significant degradation as indicated by the ability to detect even low abundant RNA species such as IFN-γ and iNOS (Fig 2). These mRNAs potentially can be characterized in a variety of ways including assessing the presence of cytokine mRNA for various interleukins as was done in this report. The exact species of mRNA probes used in these experiments was determined in part by the necessity for using probes of
References (27)
- et al.
Quantization of dendritic cells in normal and abnormal human epidermis using monoclonal antibodies directed against Ia and HTA antigens
J Invest Dermatol
(1983) - et al.
Epidermal keratinocyte production of interferon-gamma immunoreactive protein and mRNA is an early event in allergic contact dermatitis
J Invest Dermatol
(1996) - et al.
Epidermal keratinocytes production of interferon gamma immunoreactive protein and mRNA is an early event in allergic contact dermatitis
J Invest Dermatol
(1996) - et al.
Perturbation of epidermal barrier function correlates with initiation of cytokine cascade in human skin
J Am Acad Dermatol
(1994) - et al.
Epidermal cytokines IL-1β, TNFα, and IL-12 in patients with atopic dermatitis: response to application of house dust mite antigens
J Invest Dermatol
(1998) - et al.
In vivo cytokine profiles in allergic and irritant contact dermatitis
Contact Dermatitis
(1995) Contact dermatitis
Int J Dermatol
(1993)Production of cytokines by epithelial tissues: a new model for cutaneous inflammation
Am J Dermatopathol
(1989)- et al.
Recombinant gamma interferon induces HLA-DR expression on cultured human keratinocytes
Invest Dermatol
(1984) - et al.
Human keratinocytes produce mRNA for nitric oxide synthase [abstract]
J Invest Dermatol
(1995)
Nitric oxide in human skin: current status and future prospects
J Invest Dermatol
Epidermal Langerhans cells are derived from and are repopulated by mobile precursor cells which originate in bone marrow
Nature
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2022, Clinics in DermatologyCitation Excerpt :Another group introduced RNA harvesting with tape stripping.34 Using nondiseased skin irritated by sodium lauryl sulfate and nickel sulfate to induce allergic contact dermatitis, they were able to differentiate and quantify levels of IL-4 and IL-8 based on mRNA.34 This method of retrieval, RNA tape stripping, was improved by decreasing the number of tapes required to attain adequate cells, from 20 tape strips to 4, and analyzed RNA with reverse transcription-polymerase chain reaction.18
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Reprint requests: Vera B. Morhenn, MD, Clinical Associate Professor, University of California at San Diego, California Skin Research Institute, 15222-B Avenue of Science, San Diego, CA 92128.
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