Background and aims To investigate the effect of Bryophyllum pinnatum extract to B cells by supressing maturation and increasing apoptosis of B cells.
Methods In silico study was done to understand affinity of Bryophillin A, Bryophillin B, Bryotoxin A, Bryotoxin B, Kaempferol, p-coumaric acid aiffinity in Bryophyllum pinnatum against B cell activating factor (BAFF) and its receptors: B cell activating factor receptors (BAFF-R), transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), and B-cell maturation antigen (BCMA). In vitro study was done by culturing B cells from pristane induced lupus BALB/c mice’s spleen. B cells were cultured into four groups, one as a control and the other groups given Bryophyllum pinnatum extract (0.02, 0.1, and 0.5 ug/L). Precentage of maturation (CD19+/CD38+) and apoptosis (Annexin V+/PI+) of B cells determined using flow cytometry. Immunocytochemistry assay was performed to determine the expression of transcription factor NF-kB p65.
Results Molecular docking results show that all of Bryophyllum pinnatum compounds studied have affinities for BAFF-R, TACI, BCMA and BAFF. Flow cytometry assay showed significantly decrease in percentages of maturation of B cells in all doses (p<0.05), significantly increase in percentages of apoptotic B cells in dose 0.5 ug/L (p<0.05). The results of immunocytochemistry showed decreased expression of NF-kB p65 were significant at all doses (p<0.05).
Conclusions In silico and in vitro study revealed that Bryophyllum pinnatum is potential natural product which may be used for B cell depleting agent in development of biosimilar therapy in systemic lupus erythematosus.
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