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94 Development of therapy using bryophyllum pinnatum to decrease maturation and increase apoptotic b cells from balb/c lupus mice: in silico and in vitro study approach
  1. K Handono1,
  2. TWI Dantara2,
  3. E.S Dewi3,
  4. MZ Pratama4 and
  5. N Nurdiana5
  1. 1Faculty of Medicine Brawijaya University, Clinical Pathology, Malang, Indonesia
  2. 2Faculty of Medicine Brawijaya University, Biomedical Science, Malang, Indonesia
  3. 3Faculty of Medicine Brawijaya University, Medical Surgeon Nursing, Malang, Indonesia
  4. 4Faculty of Medicine Brawijaya University, Internal Medicine, Malang, Indonesia
  5. 5Faculty of Medicine Brawijaya University, Clinical Pharmacology, Malang, Indonesia

Abstract

Background and aims To investigate the effect of Bryophyllum pinnatum extract to B cells by supressing maturation and increasing apoptosis of B cells.

Methods In silico study was done to understand affinity of Bryophillin A, Bryophillin B, Bryotoxin A, Bryotoxin B, Kaempferol, p-coumaric acid aiffinity in Bryophyllum pinnatum against B cell activating factor (BAFF) and its receptors: B cell activating factor receptors (BAFF-R), transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), and B-cell maturation antigen (BCMA). In vitro study was done by culturing B cells from pristane induced lupus BALB/c mice’s spleen. B cells were cultured into four groups, one as a control and the other groups given Bryophyllum pinnatum extract (0.02, 0.1, and 0.5 ug/L). Precentage of maturation (CD19+/CD38+) and apoptosis (Annexin V+/PI+) of B cells determined using flow cytometry. Immunocytochemistry assay was performed to determine the expression of transcription factor NF-kB p65.

Results Molecular docking results show that all of Bryophyllum pinnatum compounds studied have affinities for BAFF-R, TACI, BCMA and BAFF. Flow cytometry assay showed significantly decrease in percentages of maturation of B cells in all doses (p<0.05), significantly increase in percentages of apoptotic B cells in dose 0.5 ug/L (p<0.05). The results of immunocytochemistry showed decreased expression of NF-kB p65 were significant at all doses (p<0.05).

Conclusions In silico and in vitro study revealed that Bryophyllum pinnatum is potential natural product which may be used for B cell depleting agent in development of biosimilar therapy in systemic lupus erythematosus.

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