Article Text

Download PDFPDF

2003 Tissue-resident, extravascular monocytic like cell is critical for inflammation in the synovium Perlman, Harris


Background In recent years, our understanding of the mononuclear phagocyte system has expanded, highlighting previously unknown complexities in cell origin and function. However, to date few studies have examined a role for monocytes in tissues, with the majority of studies centered on circulating monocytes, or monocyte-derived macrophages. While transcriptional studies have exposed critical gene signatures for classical monocytes (CM) and non-classical monocytes (NCM) in the bone marrow and circulation, no such studies examined heterogeneity and function at the tissue level.

Methods We utilized functional genomic analysis of murine and human synovium including single cell-CITE and ATAC seq.

Results Here, we identify and characterize intravascular (i.v.) and extravascular (e.v.) synovial monocyte populations (Syn Ly6c- cells) which are distinct in surface marker expression and transcriptional profile from circulating monocytes, dendritic cells and tissue macrophages, and are conserved in in patients with rheumatoid arthritis. e.v. Syn Ly6c- cells are independent of NR4A1 and CCR2, long-lived and embryonically derived while the i.v. Syn Ly6c- cells are dependent on NR4A1, short lived and derived from circulating monocytes. e.v. Syn Ly6c- cells undergo increased proliferation and reverse diapedesis dependent on LFA1 in response to arthrogenic stimuli and are required for the development of inflammatory arthritis.

Conclusions These findings uncover a new facet of mononuclear cell biology and are imperative to understanding tissue-resident myeloid cell function in the synovium.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: .

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.