Article Text

Download PDFPDF

293 Effect of high dose cholecalciferol during sle flares in mir-146a expression, regulatory t-cells and il-17a expression
  1. A Marinho1,
  2. C Carvalho2,
  3. D Boleixa2,
  4. A Bettencourt2,
  5. B Martins da Silva2 and
  6. C Vasconcelos1
  1. 1Centro Hospitalar Do Porto, Internal Medicine, Porto, Portugal
  2. 2ICBAS, Immunogenetics, Porto, Portugal


Background and aims A few microRNAs have known gene expression regulatory roles in innate immunity. The miR-146a, seems to be a negative regulator of innate immunity. Interestingly, miR-146a has been reported to be downregulated in PBMCs of SLE patients, being negatively correlated with clinical disease activity and with IFN levels. The ability of vitamin D to regulate miRs and their emerging relationship have been proposed through several experimental approaches. The aim of this study was to determine the Vitamin D effect in miR-146a expression and in T-Reg and TCD4+ IL-17A producing cells, in SLE.

Methods An interventional study with 3 weeks follow-up of SLE patients with a high dose vitamin D supplementation (50.000 UI or 100.000 UI/Week) was done. We assessed four female patients who had a SLEDAI >6 and at least one BILAG A. At screening, relevant data were compiled: SLEDAI-2K, BILAG score, concomitant therapy, previous SLE manifestations, 25(OH)D levels, T-Reg/IL-17A ratio and miR-146a expression. At Week 3: 25(OH) D levels, T-Reg/IL-17A+ ratio, miR-146a expression, SLEDAI 2 K, BILAG and concomitant therapy.

Results No significant difference were found, regarding Vitamin D levels, before and after supplementation. Regarding Tregs/IL-17A ratio before and after supplementation, no benefits, regarding enhancing of T-regs or decrease of Il 17-A producing cells. No significative differences were found in miR-146a expression between controls and SLE active patients before and after vitamin D supplementation.

Conclusions Severe SLE activity may cause resistance to Vitamin D therapeutical effects, including enhancing of Vitamin D levels and immunogenetic effects.

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.